摘要
背景:脂肪源性干细胞具有自我更新及多向分化潜能,在体外适当的诱导条件下可向脂肪细胞、成骨细胞、软骨细胞、心肌细胞、神经细胞和肝细胞等多种细胞分化,在组织工程中具有良好的应用前景。目的:了解脂肪源性干细胞的生物学特征及其在组织工程中的应用。方法:以"tissue-derived stem cells,tissue engineering,脂肪,间充质干细胞,组织工程"为关键词检索Elsevier数据库2000-01/2010-05与中国期刊全文数据库2000-01/2010-05相关文章。结果与结论:脂肪源性干细胞增殖速度快,取材方便、材料来源广,并且能自体取材,避免了免疫排斥问题。目前尚未找到鉴定脂肪源性干细胞的金标准,但研究者采用流式细胞仪和免疫组织化学方法研究发现体外培养的脂肪源性干细胞具有间充质干细胞这一类细胞的特异性表面标记。脂肪源性干细胞可向脂肪、骨、软骨、肌肉、造血、肝和神经等多种细胞分化。组织或器官缺损性疾病、退行性疾病及遗传性疾病可尝试通过组织工程技术将组织来源的干细胞与支架材料复合移植入体内,来解决这一临床难题已成为研究热点。
BACKGROUND：Adipose tissue-derived stem cells possess self-renewal and multi-directional differentiation potential.In vitro it could be induced to different cells in specific differentiation medium,such as lipocytes,skeltogenous cells,cartilage cells,cardiac muscle cells,nerve cells and liver cells.These characteristics show that adipose tissue-derived stem cells possess favorable application prospect.OBJECTIVE：To understand the biological characteristics of adipose-derived mesenchymal stem cells,and to explore its clinical application in tissue engineering field.METHODS：The databases of Elsevier（2000-01/2010-05） and China National Knowledge Infrastructure（2000-01/2010-05） were searched with key words of ＂adipose tissue-derived stem cell,tissue engineering＂.Literature search was limited to English and Chinese languages.RESULTS AND CONCLUSION：Adipose-derived stem cells could proliferate rapidly,and material was convenient to harvest.It could be obtained by itself,and also avoid immunological rejection.At present,there was no gold standard to identify adipose-derived stem cells,but investigators had observed in vitro cultured adipose-derived stem cells that had the specific surface labeling of mesenchymal stem cells using flow cytometry and immunohistochemical method.Adipose-derived stem cells could differentiate into adipocytes,osteocytes,chondrocytes,muscle cells,hematopoietic cells,liver cells and nerve cells.Tissue or organ defect disease,degenerative disease and hereditary disease can use tissue engineering technique to implant tissue-derived stem cells and stents into the bodies,which has been a research focus in clinic.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2010年第49期9290-9293,共4页
Journal of Clinical Rehabilitative Tissue Engineering Research
