摘要
背景:近年来有研究认为骨髓间充质干细胞可发生恶性转化,成为恶性肿瘤的细胞起源。目的:综述近年来诱导骨髓间充质干细胞分化的方法、定向分化的细胞种类及其恶性转化的相关研究进展。方法:应用计算机分别以"bone marrow mesenchymal stem cells"AND"differentiation"和"bone marrow mesenchymal stem cells"AND"transformation"为关键词检索Pubmed数据库(http://www.ncbi.nlm.nih.gov/pubmed)、SPRINGER数据库(http://www.springerlink.com)及Elsevier数据库(http://www.sciencedirect.com),时间限定为2000-01/2010-11,语言种类为English。通过阅读标题和摘要进行初筛,排除重复性研究、Meta分析,选择近期发表或发表在权威杂志的31篇相关文献进行综述。结果与结论:体外诱导骨髓间充质干细胞分化的方法主要有特定条件培养液、细胞共培养和损伤病理模型组织细胞或血清3大类。骨髓间充质干细胞可定向诱导分化为内、中及外胚层来源的几乎所有组织类型的细胞。有实验研究及临床病例个案报道,骨髓间充质干细胞可发生恶性转化,成为多种肿瘤的细胞起源。这提示骨髓间充质干细胞临床应用的安全性问题值得进一步深入研究,同时这也可能为肿瘤生物学研究提供了新视野。
BACKGROUND：Previous studies have demonstrated that bone marrow mesenchymal stem cells（BMSCs） can undergo malignant transformation and become the origin of malignant tumor cells.OBJETIVE：To review the research progress in the methods and directions of induced differentiation and malignant transformation of BMSCs in recent years.METHODS：The Pubmed database（http：//www.ncbi.nlm.nih.gov/pubmed）,SPRINGER database（http：//www.springerlink.com） and Elsevier atabase（http：//www.sciencedirect.com） were searched online with the Key words of ＂mesenchymal stem cells＂ AND ＂differentiation＂ or ＂bone marrow mesenchymal stem cells＂ AND ＂transformation＂ in English.Papers were included from January 2000 to November 2010,Language was limited to English.Following reading titles and abstracts,repetitive researches and Meta analysis were excluded,31 related articles recently published or in authoritative journals were reviewed.RESULTS AND CONCLUSION：There are 3 approaches can be used for inducing BMSCs to differentiate in vitro at least,such as certain conditioned induction mediums,co-culture with purpose cells and cells or serum derived from pathological damaged model.BMSCs can be directionally differentiationed into almost all human tissue types＇ cells that from the endoderm,mesoderm and ectoderm.Experimental researchs and clinical case reports suggest that BMSCs can undergo malignant transformation and become the cell origin of many tumors.It is indicated that the security problems of BMSCs in clinical application need further study,and it may also provide a new vision for future tumor biological research.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2011年第14期2615-2618,共4页
Journal of Clinical Rehabilitative Tissue Engineering Research
基金
江西省研究生创新专项资金(YC09A017)~~
