摘要
目的:建立复方降糖滴丸的HPLC指纹图谱,为科学评价及有效控制制剂的质量提供有效可靠的方法。方法:采用HPLC梯度洗脱方法对样品进行分离分析,色谱柱:BDS C18(4.6 mm×250 mm,5μm),流动相水-乙腈梯度洗脱,检测波长238 nm,柱温30℃。结果:10批样品得到的色谱指纹图谱有5个共有峰,相似度均在0.99以上。方法精密度、稳定性和重复性良好。结论:本方法为复方降糖滴丸的质量评价提供了依据。
Objective：To establish a sensitive HPLC fingerprint method for controlling the quality of compound Jiangtang dripping pills（CJDP）.Method：HPLC analysis was performed on a Hypersil BDS C18 column（4.6 mm × 250 mm,5 μm）,with a mixture of acetonitrile and water as mobile phase in gradient mode.The wavelength was set at 238 nm,and column temperature was 30 ℃.Result：Five peaks were identified in the HPLC fingerprint of CJDP and their similarities were more than 0.99.Good precision,stability and repetition were showed.Conclusion：This method provides an evidence to quality evaluation of CJDP.
出处
《中国实验方剂学杂志》
CAS
北大核心
2011年第8期79-81,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
浙江省科技厅社会发展重点项目(2007C23019)
