摘要
红枣经过浸泡、打浆、酶解、压滤、树脂吸附、甲酸洗脱后，采用150nm纳滤膜在压差为0．5-1MPa下过滤，结果表明：纳滤截留组分经过紫外扫描在波长258nm处峰值与环磷酸腺苷（cAMP）标准品相同，而纳滤液组分（弃去液）在波长258nm处无峰；又经过HPLC检测纳滤截留组分与环磷酸腺苷（cAMP）标准品出峰时间相同，而纳滤液组分（弃去液）在此时间处无峰。表明纳滤截留目标产物环磷酸腺苷（cAMP）效果明显，分离了大分子组分及小分子组分，纳滤后利于浓缩、纯化得到环磷酸腺苷（cAMP）成品。
Nanofihration was used in the extraction of cAMP from Jujube. Jujube was first soaked , then through puree, enzyme hydrolysis, filtration, resin adsorption and formic acid elution. The results showed： Nanofiltration fraction have a peak at a wavelength of 258nm by UV which was the same as the cyclic adenosine monophosphate （cAMP） standard; the Nanofihration discard part has no peak at 258nm; HPLC showed that Nanofiltration interception fraction has the same retention time as the cyclic adenosine monophosphate （cAMP） reference, while the Nanofiltration discard liquid do not have the peak at the same time point. This indicated that nanofihration intercepted the target product of cyclic adenosine monophosphate （cAMP） , successfully separated molecular , purified and concentrated cAMP.
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2011年第2期186-190,共5页
Food and Fermentation Industries
基金
天津市农业科技成果转化与推广计划项目(0702170)
关键词
纳膜过滤
截留组分
红枣
环磷酸腺苷
浓缩
nanofiltration, Intercept fraction, jujube, cAMP, concentrate
