摘要
以提取绿原酸后的葵花籽粕为原料,在单因素试验的基础上,采用L9(34)正交优化试验确定葵花籽粕蛋白最佳制备工艺。采用蛋白层析仪分离纯化葵花籽粕蛋白,确定了纯化工艺,同时以大豆蛋白为对照,研究了葵花籽粕蛋白的溶解性、起泡性与起泡稳定性、乳化性与乳化稳定性、持水性、持油性等功能性质。结果表明,葵花籽粕粗蛋白最佳碱提工艺为料液比1∶25(g/m L)、温度40℃、时间30 min、碱液p H 12.0,提取率为61.04%±2.5%,蛋白质含量为66.94%±3.2%;分离纯化的工艺条件为上样量3.0 m L、洗脱液流速0.4 m L/min,纯化后蛋白质含量为93.20%±3.5%,纯度提高了26.36%±1.6%;葵花籽粕蛋白的等电点PI为4.2;葵花籽粕蛋白的溶解性,起泡性、起泡稳定性、乳化性、乳化稳定性均略高于大豆蛋白,持水性略低于大豆蛋白,持油性明显高于大豆蛋白。葵花籽粕蛋白有望作为食品工业的风味保持剂。
With sunfl ower seed meal extracted chlorogenic acid as raw material,On the basis of single factor,the orthogonal experiment of L9(3^4)was used to determining the best preparation technology of sunfl ower seed meal protein(SSMP).SSMP was separated and purifi cated by using protein chromatography instrument,and the purification parameters was determined.At the same time,the functional properties of solubility,foamability,foaming stability,emulsifying,emulsifying stability,water holding and oil binding capacity of SSMP were analyzed with soybean protein as control.The results showed that the optimal lye extraction process were as follows:ratio of material to liquid 1∶25(g/mL),temperature 40℃,time 30 min,pH value of alkali solution 12.0,and under these conditions,extraction rate was up to 61.04%±2.5%,protein content reached 66.94%±3.2%.Moreover,the separation and purification technics of SSMP were as follows:sample volume 3.0 mL,fl ow rate of eluent 0.4 mL/min,and protein content reached 93.20%±3.5%while purity was increased by 26.36%±1.6%.The isoelectric point of SSMP was PI=4.2.The solubility,foamability,foaming stability,emulsifying and emulsifying stability of SSMP were all higher than that of soybean protein while water-holding capacity was slightly lower.In addition,holding oil capacity was significantly higher than that of soy protein.SSMP is expected to serve as fl avor retaining agent for applying to food industry.
作者
乔砥
敬思群
岳海涛
QIAO Di;JING Si-qun;YUE Hai-tao(College of Life Sciences and Technology,Xinjiang University,Urumqi 830046,Xinjiang,China;Yingdong Institute of Food Science and Engineering,Shaoguan University,Shaoguan 512005,Guangdong,China)
出处
《粮食与油脂》
北大核心
2018年第9期34-39,共6页
Cereals & Oils
基金
国家自然科学基金(31660462)
关键词
葵花籽粕蛋白
提取与纯化
功能性质
sunfl ower seed meal protein(SSMP)
extraction and purifi cation
functional property
