摘要
目的 :建立同时测定血清中苯巴比妥、苯妥英钠和卡马西平的浓度的高效液相色谱法 (HPLC)。方法 :血清乙酸乙酯提取浓集后进样 ,采用C18柱 ( 2 2 0× 4 .6,5μm) ,以甲醇 -水 ( 50∶50 )为流动相 ,苯乙酮为内标物 ,在波长2 1 0nm处测定。结果 :苯巴比妥、苯妥英钠和卡马西平的线性范围为 0 .63～ 4 4μg/ml,三者平均回收率均大于 97% ,日内和日间误差小于 5%。结论 :本法快速、简便、准确 ,适用于治疗药物监测和药动学研究。
OBJECTIVE:To simultaneously determine phenobarbital,phenytoin and carbamazepine in serum by HPLC.METHODS:Acetophenone was selected as the internal standard.A UV detector(λ210nm) and C 18 column was used.Serum sample(0.1ml) was extracted with 1ml acetic ether.The organic layer was evaporated to dryness under N 2 stream in at water bath 60℃.The residue was dissolved with 100μl acetophenone and 20μl was injected.The mobile phase was methanol water(50∶50).RESULTS:The linearrange of phenobarbital,phenytoin and carbamazepine was 0.63～44μg/ml.The RSD of inter-day and intra-day in three substances was less than 5%.The extraction recovery was more than 97%.The detection limits in serum was 0.25μg/ml for phenobarbital,phenytoin and carbamazepine.CONCLUSION:The method was accurate and sensitive,suitable for TDM and pharmacokinetic study of phenobarbital,phenytoin and carbamazepine.
出处
《中国现代应用药学》
CAS
CSCD
1999年第4期41-43,共3页
Chinese Journal of Modern Applied Pharmacy
