摘要
目的检测促红细胞生成素产生肝细胞受体A2（EphA2）蛋白在喉鳞癌组织及细胞系中的表达，探讨其表达与喉鳞癌临床病理特征和预后之间的关系。方法采用Western blot技术，检测EphA2蛋白在喉鳞癌细胞株Hep-2及头颈部永生化上皮细胞株NP-69中的表达；采用免疫组织化学技术，检测EphA2蛋白在88例喉鳞癌及16例癌旁黏膜组织中的表达，并分析EphA2与临床病理特征和预后的关系。结果EphA2蛋白在Hep-2细胞株中的表达高于NP-69细胞株。EphA2蛋白在喉鳞癌组织及癌旁黏膜组织中的阳性表达率分别为80．7％和43．8％，差异有统计学意义（P〈0．001）。EphA2高表达与喉鳞癌的临床分期（P=0．005）、淋巴结转移（P=0．025）和复发（P=0．021）密切相关。Kaplan—Meier生存分析结果显示，EphA2高表达组与低表达组的5年无瘤生存率分别为33．3％和63．2％，5年生存率分别为46．7％和81．6％，差异有统计学意义（P=0．003，P=0．002）。EphA2表达水平结合临床分期对喉鳞癌预后具有更好的预测价值。单因素及多因素Cox比例风险回归模型进一步显示，EphA2表达水平为喉鳞癌预后的独立影响因素（P=0．019）。结论EphA2蛋白在喉鳞癌组织和细胞中的表达显著升高，且其高表达与喉鳞癌患者的复发、转移及预后密切相关。EphA2可能在喉鳞癌的发生、发展中有重要作用，有望成为评估喉鳞癌复发、转移及预后的重要分子标志物。
Objective To evaluate the expression of EphA2 protein in tissue specimens and cell lines of laryngeal squamous cell carcinoma （LSCC） , and to further study the correlation of EphA2 protein expression with clinicopathological characteristics and prognosis in LSCC. Methods Western blot was applied to assess the EphA2 protein expression in LSCC cell line Hep-2 cells and the head and neck immortalized epithelial cell line NP-69 cells. Immunohistochemical staining was performed on paraffin sections of 88 cases of LSCC specimens and 16 cases of adjcent normal tissue samples to investigate the EphA2 protein expression, and to luther elucidate its correlation with clinicopathological characteristics. Results Compared with the NP-69 cells, EphA2 expression in LSCC cell line Hep-2 cells was upregulated. The positive rates of EphA2 expression in I-SCC and adjcent normal tissues samples were 80.7% and 45.8%, respectively, with a significant difference between the two groups （P 〈 0. 001 ）. EphA2 overexpresion was closely correlated with clinical stage （ Ⅰ ＋ Ⅱ /Ⅲ ＋ Ⅳ, P = 0. 005 ） , metastasis （ P = 0. 025 ） and recurrence（P = 0. 021 ） in LSCC. Furthermore, patients with EphA2 overexpression had poorer tumor-free survival and 5-year overall survival compared with that in patients with low EphA2 expression （33.3% vs.63.2%, P = 0. 003; 46.7% vs. 81.6%, P = 0. 002 ）. EphA2 expression combined with clinical stage provided a better predictive value in prognosis. Univariate and multivariate Cox regression analysis revealed that EphA2 expression is an independent prognostic factor for patients with LSCC（P = 0.019）. Conclusions The results of this study demonstrate that EphA2 protein expression is significantly increased in LSCC tissues and cell lines, and EphA2 protein overexpression is associated with tumor recurrence, metastasis and poorer prognosis in LSCC patients. These results suggest that EphA2 may play a critieal role in the initiation and progression of LSCC, implicating EphA2 as a v
出处
《中华肿瘤杂志》
CAS
CSCD
北大核心
2011年第3期197-201,共5页
Chinese Journal of Oncology
基金
国家自然科学基金（81071757、30901664、30872852）
高等学校博士学科点专项科研基金（20100162110036、20090162110065）
湖南省科技厅项目（2007SK2003、2007SK3050）
湖南省研究生学位论文创新基金（CX2010B053）.志谢 中南大学湘雅医院病理科李波老师和医学研究中心冯雪萍老师对本实验的悉心指导和帮助
关键词
喉肿瘤
肿瘤转移
复发
预后
EPHA2
Laryngeal neoplasms
Neoplasm metastasis
Recurrence
Prognosis
EphA2
