摘要
目的比较全身炎症反应综合征(SIRS)患儿与健康儿童血清IL-6、inter-α抑制蛋白(IαIP)水平,并动态观察IαIP/IL-6比值在SIRS发展过程中的变化。方法根据SIRS的诊断标准,将本院儿科收治的38例SIRS患儿纳入SIRS组,同期选取22例健康儿童作为健康对照组,采用Western blot法检测其血清IαIP水平,并对其条带进行灰度扫描,采用ELISA法测定其血清IL-6水平,比较SIRS组与健康对照组血清IL-6、IαIP水平。参照小儿各系统功能障碍诊断标准,将住院过程中新出现1个以上器官功能障碍者作为恶化组,并将SIRS组治疗痊愈或好转的患儿作为治愈/好转组,病情恶化或死亡的患儿作为恶化/死亡组,比较2组血清IL-6、IαIP水平及IαIP/IL-6比值;在恶化/死亡组中比较恶化后与入院时血清IL-6、IαIP水平和IαIP/IL-6比值。结果 1.SIRS组血清IL-6水平明显高于健康对照组(P<0.01),而SIRS组血清IαIP水平明显低于健康对照组(P<0.01)。2.病情恶化/死亡组与治愈/好转组相比,血清IL-6水平显著升高(P<0.01),而血清IαIP水平、IαIP/IL-6比值均显著降低(Pa<0.01)。3.病情恶化组患儿病情恶化后与入院时比较,血清IL-6水平显著升高(P<0.01),而血清IαIP水平、IαIP/IL-6比值均显著降低(Pa<0.01)。结论血清IL-6、IαIP水平与IαIP/IL-6比值的高低可判断SIRS的预后。
Objective To detect the levels of serum interleukin-6（IL-6） and inter-α inhibitor protein（IαIP） in children with systemic inflammatory response syndrome（SIRS） and compare with healthy children,and to observe the changes of IαIP/IL-6 ratio dynamically during the progress of SIRS.MethodsAccording to the diagnostic criteria of SIRS,38 patients with SIRS admitted into the Department of Pediatrics of the Affiliated Hospital of Nantong University were put into SIRS group while 22 healthy children were put into healthy control group.The level of serum IαIP was determined by using Western blot and the gray value of the strap was recorded,while the level of serum IL-6 was detected by using enzyme-linked immunosorbent assay.The levels of serum IL-6 and IαIP were compared between SIRS group and healthy control group.According to the diagnostic criteria of system dysfunction of children,the patients with more than one new organ dysfunction during the admission were put into worsening group.Then the patients were either put into healing/better group or into worsening/death group within SIRS group.The levels of serum IL-6,IαIP and ratio of IαIP/IL-6 were compared between the 2 groups.The levels of serum IαIP and ratio of IαIP/IL-6 were compared at admission and after deterioration in worsening/death group.Results1.The level of serum IL-6 in SIRS group was significantly higher than that in healthy control group（P0.01）.While the level of serum IαIP in SIRS group was significantly lower than that in healthy control group（P0.01）.2.The level of serum IL-6 increased significantly（P0.01） in wor-sening/death group,while the levels of serum IαIP and ratio of IαIP / IL-6 decreased significantly（Pa0.01） compared with healing/better group.3.The level of serum IL-6 increased significantly（P0.01）after deterioration,compared with admission time（P0.01） in worsening group,while the IαIP and IαIP/IL-6 ratio decreaced significantly（Pa0.01）.ConclusionThe levels of serum IL-6,IαIP and IαIP/IL
出处
《实用儿科临床杂志》
CAS
CSCD
北大核心
2011年第6期418-419,422,共3页
Journal of Applied Clinical Pediatrics
基金
南通大学自然科学项目(08Z051)
