摘要
目的探讨慢性铝暴露对小鼠认知能力的影响及与脑组织β-淀粉样前体蛋白(β-APP)、β-淀粉样蛋白(β-AP)表达的关系。方法动物模型高、中、低剂量组每日用AlCl3含量120、12、1.2 mg/kg饲料进行喂养,对照组喂饲正常饲料,染毒9个月后用Morris水迷宫实验、跳台实验、避暗实验进行行为学测试,取脑,观察小鼠组织学变化,用蛋白质印迹(western-blot)法检测小鼠脑组织中β-APP和β-AP蛋白的表达。结果对照组小鼠跳台实验潜伏期与错误次数为(205.00±30.09)s和(0.29±0.49)次,铝暴露中、高剂量组跳台实验潜伏期与错误次数分别为(159.63±59.61)、(103.02±41.86)s和(1.63±1.06)、(2.00±1.63)次,与对照组比较,潜伏期明显缩短,错误次数明显增加(P<0.05);与对照组避暗实验潜伏期(265.57±15.05)s比较,铝暴露中、高剂量组小鼠避暗潜伏期〔(126.71±25.73)、(45.57±33.13)s〕明显缩短(P<0.05);与对照组比较,铝暴露中、高剂量组β-APP、β-AP蛋白表达量均明显增多(P<0.05,P<0.01)。结论慢性铝暴露可导致小鼠β-APP和β-AP的表达上调,这可能是铝致小鼠认知功能障碍的重要机制之一。
Objective To explore the effect of chronic aluminium exposure on cognitive ability in mice and the relationship with the expressions of β-amyloid precursor protein（β-APP）and β-amyloid protein（β-AP）. Methods Animal models of high,moderate and low dose aluminium exposure were established with AlCl3 in the feed at daily content of 120,12,and 1.2 mg/kg.Cognitive abilities were evaluated with step down test,Morris water maze（MWM） and passive avoidance test after 9 months′ exposure.The expressions of brain β-APP and β-AP were detected with western-blot. Results Step down test latency and the number of errors was 205.00±30.09 seconds and 0.29±0.49 time for the mice in control group,and those of moderate and ghigh dose aluminium exposure group were 159.63±59.61,103.02±41.86s and 1.63±1.06,2.00±1.63 times,respectively.Compared with the control group,the incubation period decreased and the number of error increased significantly in exposure groups（P0.05）.Compared with the control group,β-APP,β-AP expressions were significantly increased in the mice with high dose aluminium exposure（P0.05,P0.01）. Conclusion Chronic aluminium exposure can lead to cognitive dysfunction in mice.The increased expression of β-APP and β-AP may be an important mechanism.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2011年第4期461-462,共2页
Chinese Journal of Public Health
基金
国家自然科学基金(30740032)
