摘要
目的研究4种不同种类的铝化合物对大鼠肾上腺髓质嗜铬瘤分化细胞株PC12细胞的毒性作用。方法取对数生长期PC12细胞,分别予麦芽酚铝(染毒剂量为0.0、0.1、0.2、0.4、0.8 mmol/L)、三氯化铝(染毒剂量为0.0、1.0、2.0、4.0、8.0 mmol/L)、柠檬酸铝(染毒剂量为0.0、1.0、2.0、4.0、8.0 mmol/L)和乳酸铝(染毒剂量为0.0、2.0、4.0、8.0、16.0 mmol/L)染毒24 h。采用CCK-8法检测细胞活力,以流式细胞术检测细胞凋亡率。结果随着4种铝化合物染毒剂量的增加,PC12细胞存活率均下降,细胞凋亡率均上升,均呈剂量-效应关系(P<0.01)。对于麦芽酚铝、三氯化铝、柠檬酸铝和乳酸铝4种铝化合物,与对照组比较,导致PC12细胞活力出现有统计学意义下降的最低作用剂量(MEC)分别为0.2、2.0、2.0和4.0 mmol/L(P<0.05),导致PC12细胞凋亡率出现有统计学意义升高的MEC分别为0.1、2.0、1.0和2.0 mmol/L(P<0.05)。麦芽酚铝、三氯化铝、柠檬酸铝、乳酸铝对PC12细胞活力的24 h-半数抑制浓度分别为(0.45±0.01)、(4.02±0.39)、(5.37±0.88)和(6.31±0.58)mmol/L。结论 4种铝化合物均可引起PC12细胞的细胞活力下降和凋亡率升高,对PC12细胞均具有细胞毒性作用,且呈剂量-效应关系;但麦芽酚铝在较低剂量即对PC12细胞产生明显的剂量依赖性毒作用,以麦芽酚铝进行铝的离体神经毒性研究比其他铝化合物更具有优势。
Objective To investigate the toxic effects of four different kinds of aluminum compounds in rat adrenal-derived pheochromocytoma cell PC12. Methods PC12 cells at logarithmic growth phase were treated with four different kinds of aluminum compounds： aluminum maltolate（ concentration was 0. 0,0. 1,0. 2,0. 4,0. 8 mmol / L）,aluminum chloride（ concentration was 0. 0,1. 0,2. 0,4. 0,8. 0 mmol/L）,aluminum citrate（ concentration was 0. 0,1. 0,2. 0,4. 0,8. 0mmol / L） and aluminum lactate（ concentration was 0. 0,2. 0,4. 0,8. 0,16. 0 mmol / L） for 24 hours,respectively. The cell viability was determined with CCK-8 assay,and the apoptotic rate was detected by flow cytometry. Results All of the aluminum compounds suppressed the cell viability and increased apoptosis（ P〈0. 01）. Most of the effects were in a dose dependent manner. Comparing with the control,the minimum effective concentration of aluminum maltolate,aluminium chloride,aluminum citrate and aluminum lactate were 0. 2,2. 0,2. 0 and 4. 0 mmol / L,respectively,in cell viability（ P〈0. 05）; and 0. 1,2. 0,1. 0 and 2. 0 mmol/L in cell apoptosis（ P〈0. 05）. The 24 hours 50% inhibitory concentration of the above four aluminum compounds were（ 0. 45 ± 0. 01）,（ 4. 02 ± 0. 39）,（ 5. 37 ± 0. 88） and（ 6. 31 ±0. 58） mmol / L,respectively. Conclusion Treatment of all four aluminum compounds had reduced cell viability and increased the percentage of cell apoptosis in a dose-dependent manner in PC12 cells. The best dose-response relation was observed in aluminum maltolate treatment group,and a relatively low dose of it was required in in-vitro toxicology study.Therefore,aluminum maltolate posed to be better reagent than the other three for in-vitro aluminum toxicity study.
出处
《中国职业医学》
CAS
北大核心
2017年第1期20-24,共5页
China Occupational Medicine
基金
国家自然科学基金(81430078
81372968)
