摘要
背景:丹参被认为有防治皮瓣缺血再灌注损伤的作用,但其具体机制尚不明确。目的:观察丹参注射液对皮瓣缺血再灌注损伤的保护作用,并探讨其机制。方法:48只Wistar大鼠随机分为3组(n=16):对照组:不进行灌注干预;缺血再灌注组:术前3d至术后5d,每日腹腔注射生理盐水2mL/kg;丹参组:将生理盐水换为等量的丹参注射液。在大鼠以腹壁浅血管为蒂的岛状皮瓣缺血再灌注模型上,分别测定皮瓣组织中丙二醛、髓过氧化物酶、细胞间黏附分子1、核因子κB含量,并对切片进行图像分析,观察皮瓣成活率。结果与结论:缺血再灌注组、丹参组于再灌注后2,8,24h,对照组于术后10,16,32h,丙二醛、髓过氧化物酶值以及核因子κB和细胞间黏附分子1的表达均呈现出:缺血再灌注组>丹参组>对照组(P<0.01)。皮瓣成活率为:缺血再灌注组<丹参组<对照组(P<0.01)。结果提示,丹参可能通过抑制核因子κB激活环形炎症反应通路及自身即具有的抗氧化、提高机体总体抗氧化的能力,减轻皮瓣缺血再灌注损伤,促进皮瓣成活。
BACKGROUND：Salvia miltiorrhiza （SM） is considered to be helpful to prevent flap ischemia-reperfusion injury （IR）,but the specific mechanism is not clear. OBJECTIVE：To observe the protective effects of SM injection on the flap IR injury in rats and to explore its mechanism. METHODS：Totally 48 Wistar rats were randomly divided into 3 groups,with 16 animals in each group. No perfusion was performed in the control group. Physiological saline （2 mL/kg） was intraperitoneal injected into rats at 3 days before until 5 days after operation in the IR group. The same volume of SM injection was injected into rats in the SM-IR group. The contents of malonaldehyde,myeloperoxidase,intercellular adhesion molecule 1 （ICAM-1） and nuclear factor-κB of the island skin flap IR models were measured,and the survival rate of flap was observed using image analysis. RESULTS AND CONCLUSION：The expressions of malonaldehyde,myeloperoxidase,ICAM-1 and nuclear factor-κB in the IR group,SM-IR group after reperfusion 2,8,24 hours and the control group after 10,16,32 hours showed：IR group SM-IR group control group （P 0.01）. The survival rate of flap was：IR group SM-IR group control group （P 0.01）. In a conclusion,salvia miltiorrhiza may inhibit NF-κB activation pathway and its own ring-inflammatory response that has the anti-oxidation,improve the body＇s overall antioxidant capacity,reduce flap ischemia-reperfusion injury,thus,promote flap survival.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2011年第5期855-860,共6页
Journal of Clinical Rehabilitative Tissue Engineering Research
基金
河北省科学技术研究与发展计划基金资助项目(09276102D-31)
课题名称:皮瓣缺血再灌注损伤中凋亡基因的表达及其临床应用研究~~
