摘要
目的探讨阿霉素预处理提供鼠皮瓣缺血耐受的可能性及其作用机制。方法健康成年SD大鼠24只,雌雄各半,体重250～300 g,随机分为A、B、C 3组(n=8)。于各组大鼠腹部制备以腹壁浅血管-神经束为蒂、大小为6 cm×3 cm的岛状皮瓣,A组不作处理;B组用微血管夹阻断腹壁浅血管血流10 min,松开后再灌注10 min,反复4次;C组经腹壁浅静脉推注阿霉素(1 mg/kg)。24 h后于皮瓣蒂部夹闭腹壁浅血管4 h,再灌注2 h,制备缺血再灌注损伤模型。术后观察大鼠存活情况,于缺血再灌注损伤后0、8、12、24、30 h各组取皮瓣检测丙二醛(malonyldiadehyde,MDA)和超氧化物歧化酶(superoxide dismutase,SOD)含量;于缺血再灌注损伤后7 d测量皮瓣成活率后处死大鼠,取皮瓣行组织学观察。结果实验中共5只大鼠死亡,其中A、B组各1只,C组3只,均给予补充。缺血再灌注损伤后7 d,A组皮瓣成活率为10.10%±0.43%,小于B组91.63%±1.76%及C组92.75%±1.48%,差异均有统计学意义(P<0.05);B组与C组比较,差异无统计学意义(t=0.29,P=0.77)。缺血再灌注损伤后0 h 3组间MDA和SOD含量比较,差异均无统计学意义(P>0.05);8 h后各时间点A组与B、C组比较,差异均有统计学意义(P<0.05),B组与C组比较差异无统计学意义(P>0.05)。组织学观察示,A组炎性细胞浸润较B、C组明显,纤维增生减弱;B组与C组皮瓣组织学改变相似。结论阿霉素预处理可以提供鼠皮瓣缺血耐受,保护皮瓣减轻缺血再灌注损伤,其机制可能与诱导内源性保护物质的产生有关。
Objective To investigate the effects and mechanism of doxorubicin preconditioning in providing ischemic tolerance for rats abdomen island flaps. Methods Twenty-four healthy adult Sprague Dawley rats, 12 males and 12 females, were randomly divided into 3 groups （n=8）： control group （group A）, ischemic preconditioning group （group B）, and doxorubicin preconditioning group （group C）. After the abdomen island flap （6 cm x 3 cm in size） based on the superficial inferior epigastric neurovascular bundle was prepared, group A had no further treatment; group B was given a 10-minute ischemia followed by a 10-minute reperfusion for 4 times; and group C was given pretreatment with doxorubicin （1 mg/kg） by injection of the inferior epigastric vein. After 24 hours, the inferior epigastric vessels were blocked by vascular clamp for 4 hours, followed by reperfusion 2 hours to prepare ischemia/reperfusion （I/R） injury model. The rat survival was observed after operation; at 0, 8, 12, 24, and 30 hours after I/R injury, the malonyldiadehyde （MDA） and superoxide dismutase （SOD） levels were measured. At 7 days after I/R injury, the survival rate of flap were calculated and the flaps were harvested for histological observation. Results During experiment, 5 rats died （1 rat in groups A and B respectively, 3 rats in group C） and were added. The survival rates of the flap in group A （10.10% _＋ 0.43%） was lower than those in group B （91.63% ＋ 1.76%） and in group C （92.75% ＋ 1.48%） at 7 days after I/R injury, showing significant differences （P 〈 0.05）, and there was no significant difference between groups B and C （t=-0.29, P=0.77）. Significant difference was found in MDA level and SOD level between group A and groups B, C after 8 hours （P 〈 0.05）, and there was no significant difference between groups B and C （P 〉 0.05）. Histological observation showed that inflammatory cells infiltration was more obvious and hyperplasia of fibers was weaker in group A than
出处
《中国修复重建外科杂志》
CAS
CSCD
北大核心
2012年第12期1501-1504,共4页
Chinese Journal of Reparative and Reconstructive Surgery
关键词
阿霉素
皮瓣缺血再灌注损伤
缺血预处理
大鼠
Doxorubicin Ischemia/reperfusion injury of flap Ischemic preconditioning Rat
