摘要
目的探讨补肾中药对肾阳虚小鼠肾上腺细胞形态学的影响。方法以"劳倦过度,房室不节"造成肾阳虚小鼠模型。造模小鼠随机分成正常组、模型组、金匱肾气丸治疗组,造模4周后观察补肾中药金匱肾气丸对肾上腺细胞形态学的影响。结果 "劳倦过度、房室不节"肾阳虚小鼠较正常小鼠肾上腺质量显著下降(P(0.01)、肾上腺系数明显下降(P(0.05);光镜下可见,小鼠肾上腺皮质变薄,皮质球状带、束状带变窄、结构不清,细胞排列不规则,细胞体积缩小、染色变浅,细胞质呈空泡状,细胞间隙变宽;电镜下可见肾上腺皮质束状带细胞核形状不规则、变形,核膜受损不清晰,外层核膜突向胞质,染色质团块状,线粒体的数量比正常组显著减少,部分线粒体明显肿胀、变性,同心圆或指纹状嵴,粗面内质网较正常组少,滑面内质网显著扩张,呈小泡状。金匱肾气丸治疗组肾上腺皮质较模型组有一定程度的改善,形态接近于正常组。结论肾阳虚小鼠肾上腺组织形态学及超微结构均发生退行性改变,肾气丸治疗后可使肾阳虚小鼠异常的肾上腺细胞形态得以恢复。
Objective：To explore the effect of traditional Chinese medicine for kidney-reinforcing of morphological alterations of the adrenal gland in mice with Kidney-Yang deficiency.Methods：The model of Kidney-Yang deficiency was established by overstrain and excessive sexual intercourse.The mice were randomly divided into three groups,including the normal control group,the Kidney-Yang deficiency group,and the Jinkuishenqi bolus group.After four-week treatment of traditional Chinese medicine for kidney-reinforcing,morphological changes of the adrenal gland were observed in all groups.Result：Compared with the normal control group,the weight and coefficient of the adrenal gland in the mice with Kidney-Yang deficiency were both obviously decreased（P﹤0.01;P﹤0.05）.Under a light microscope,it was shown that in the group of Kidney-Yang deficiency,the adrenal cortex became thin,and the zona glomerulosa and fasciculate were narrow.The structure of cells was irregularly arranged,while the cellular volume was reduced with shallow staining.In addition,vacuole-shaped cytoplasm and widened intercellular space were identified.Under an electronic microscope,the shapes of nuclei were irregular in the glomerulosa zone of the adrenal cortex,while the nuclear membrane dissolved,the outer nuclear membrane protruded into the cytoplasm,and the chromatin were clumped.Compared with the normal control group,the number of mitochondria in the Kidney-Yang deficiency group was remarkably decreased,and parts of them were swollen and degenerated.The mitochondrial cristae were concentric or fingerprint-shaped and rough endoplasmic reticulum in the Kidney-Yang deficiency group were fewer than that in the normal control group. Smooth endoplasmic reticulum was obviously enlarged and vesicle-shaped.In contrast to the untreated group,the morphology of the adrenal cortex in the Jinkuishenqi bolus group was improved to a certain degree,which was similar to the normal control group.Conclusion：Morphology and ultra-structure of the adrenal gland in
出处
《山东大学学报（医学版）》
CAS
北大核心
2011年第2期67-70,共4页
Journal of Shandong University:Health Sciences
基金
国家自然科学基金重大研究计划资助项目(编号90209014)
关键词
金匱肾气丸
肾阳虚
模型
动物
肾上腺形态
小鼠
Jinkuishenqi bolus
Kidney-yang deficiency
Model
animals
Morphology
adrenal gland
Mice
