摘要
目的：介绍一种同时评估耳蜗传出神经和毛细胞的简便的组织化学技术。方法：首先应用脱氢酶染色选择性标记毛细胞，再用乙酰胆碱酯酶染色标记传出神经纤维。双重染色的耳蜗铺片样品在光学显微镜下沿着耳蜗基底膜的全长分别对毛细胞和穿越Ｃｏｒｔｉ隧道的传出神经纤维计数；根据卡铂耳中毒灰鼠的毛细胞及其传出神经纤维损伤的百分比制备耳蜗图。结果：耳蜗分析图充分显示：当绝大多数内毛细胞坏死以及部分外毛细胞坏死时，穿越隧道的传出神经纤维数的减少接近于外毛细胞损失的百分比；但当大量内毛细胞坏死而外毛细胞正常时，穿越隧道的传出神经纤维亦保持正常。结论：卡铂损害耳蜗传出神经系统可能是由于外毛细胞受损所致。
Objective:This paper describes a convenient histochemical technique that allows one to simultaneously assess the cochlear efferent neurons and inner (IHCs) and outer hair cells (OHCs).Method:Selective labeling of the sensory cells and efferent fibers was carried out by first labeling the hair cells for dehydrogenase activity and then staining the efferent fibers for acetylcholinesterase activity.Double labeled specimens were prepared as a surface preparation and counts were made of the number of hair cells and the number of discreate fiber bundles crossing the tunnel of Corti along the entire length of the cochlea.Result:The utility of this technique for assessing ototoxic damage was demonstrated by constructing cochleograms showing the percentage of missing hair cells and tunnel crossing efferent fibers in chinchillas treated with carboplatin.In the region of the cochlea associated with most IHC loss and a part of OHC missing showed by succinate dehydrogenase in the region of hair cells;however,the loss of tunnel crossing efferent fibers showed by acetylcholinesterase staining was closely correlated with the percentage of OHC loss.But,if only damage the IHC,the efferent fibers showed normal.Conclusion:These results suggest that carboplatin damaged efferent sytems may be due to the damage of OHC.
出处
《临床耳鼻咽喉科杂志》
CSCD
1999年第11期510-512,共3页
Journal of Clinical Otorhinolaryngology
