摘要
目的介绍用于颞骨病理学研究的分子生物学新技术。方法所有颞骨火棉胶切片均来自解放军总医院颞骨库,基于颞骨火棉胶切片的DNA提取、PCR扩增、原位杂交、原位PCR和免疫蛋白染色技术被用来研究老年性耳聋的分子病理机制。结果除了原位PCR技术,基于颞骨火棉胶切片的DNA提取、PCR扩增、原位杂交和免疫蛋白染色技术均取得了可接受的、有用的结果来帮助解释老年性耳聋的机制。结论存档的颞骨标本是非常珍贵的资源,加以利用可以揭示各型耳聋的分子病理机制。
Objective To review the new techniques to study the pathology of temporal bones. Methods All archival serial celloidin sections of temporal bones are stored in the temporal bone bank of PLA general hospital. DNA extraction,PCR amplification,in situ hybridization,in situ PCR and immune- histological staining based on the archival celloidin embedded section were used to reveal the pathogenesis of presbycusis. Results All new techniques except in situ PCR can yield acceptable and useful results to help explain the mechanism of presbycusis. Conclusion The whole collection of archival temporal bones is the precious resource for exploring the pathogenesis for the all kinds of hearing loss.
出处
《中华耳科学杂志》
CSCD
2004年第3期224-226,共3页
Chinese Journal of Otology
