摘要
内耳病理学是一门从组织形态学角度研究内耳疾病的发生原因和发展过程及其损害机制的边缘医学基础学科。内耳病理学的样品制备观察技术包括颞骨切片技术、膜迷路切片技术、耳蜗和前庭膜迷路取材铺片技术、内耳组织学和组织化学技术、内耳扫描电镜和透射电镜样品制备技术、以及电镜下的超微细胞化学技术等。通过分析各项内耳病理研究技术的发生、发展和现状,并结合实践经验和技术革新经验,深入讨论上述各项技术的优缺点和技术细节。
Pathology of the inner ear is a research branch of otology using morphological experimental techniques to study the causes, the development process and damage mechanisms of the inner ear diseases. The general field of pathology of the inner ear includes sectioning of the temporal bone or membranous labyrinth, cochlear and vestibular micro-dissections and surface preparations, histology and bistochemistry, preparations of membranous labyrinth for observation under scanning and transmission electron microscope, and electron microscopic cytochemistry, etc. This article has described in detail the occurrence, development and current status of above-mentioned techniques for pathological studies in the inner ear. Based on our own experiences and many years of innovative practices, the advantages and disadvantages of each technology and their technical details were also discussed.
出处
《中国耳鼻咽喉颅底外科杂志》
CAS
2016年第3期169-178,共10页
Chinese Journal of Otorhinolaryngology-skull Base Surgery
基金
上海市科学技术委员会资助(15140900900)
关键词
内耳
病理学
组织形态学
研究进展
Inner ear
Pathology
Morphological experimental technique
Study
