摘要
目的探讨黏蛋白1（MUC1）和黏蛋白2（MUC2）在乳房外Paget病（EMPD）中的表达情况。方法生物素蛋白免疫组化法（sP法）检测19例EMPD皮损及19例美容切除术后正常皮肤组织上MUC1与MUC2的表达。结果19例EMPD皮损常规HE染色显示，3例伴低分化腺癌，6例呈浸润性，10例为上皮内。MUC1在3例伴腺癌Paget病中有2例呈阳性表达，6例浸润性和10例上皮内Paget病均呈阳性表达。MUC2在3例伴腺癌Paget病和6例浸润性Paget病均呈阳性表达，在10例上皮内Paget病中有2例呈阳性表达。MUC1与MUC2在正常皮肤组织呈阴性表达。MUC1在上皮内Paget病中的表达显著高于伴腺癌Paget病和浸润性Paget病（P〈0．05）。MUC2在伴腺癌Paget病和浸润性Paget病中的表达显著高于上皮内Paget病（P〈0．05）。MUC1和MUC2的表达无明显相关性（r=-0．5，P〉0．05）。结论MUC1在EMPD中呈普遍表达，MUC2在伴有腺癌和浸润性EMPD中呈阳性表达。
Objective To study the expressions of mucin （MUC） 1 and 2 in extramammary Paget＇s disease（EMPD） lesions. Methods Tissue specimens were obtained from the lesions of 19 patients with EMPD and normal skin of 19 human controls during cosmetic surgery. Streptavidin-perosidase （SP） technique was used to detect the expressions of MUC1 and MUC2 in these specimens. Results As haematoxylin-eosin （HE） staining showed, 3 cases were accompanied by poorly differentiated adenocarcinoma, 6 were invasive Paget＇s disease and 10 were intraepithelial EMPD. MUC1 was expressed in 2 cases accompanied by poorly differentiated adenocarcinoma, and in all the cases of invasive and intraepithelial EMPD; MUC2 was observed in all the cases of adenocarcinoma-complicated EMPD and invasive EMPD, but only in 2 of 10 cases of intraepithelial EMPD. Neither MUC1 nor MUC2 was observed in normal control specimens. A significant increase was observed in the expression of MUC 1 in lesions of intraepithelial EMPD compared with invasive EMPD and adenocarcinomacomplicated EMPD （both P 〈 0.05）, and in the expression of MUC2 in lesions of invasive EMPD and adenocarcinoma-complicated EMPD compared with intraepithelial EMPD （both P 〈 0.05）. The expression of MUC1 was uncorrelated to that of MUC2 （r = -0.5, P 〉 0.05）. Conclusions MUC 1 is generally expressed in the lesions of EMPD, while MUC2 is expressed in those of adenocarcinoma-complicated EMPD and invasive EMPD.
出处
《中华皮肤科杂志》
CAS
CSCD
北大核心
2011年第3期165-167,共3页
Chinese Journal of Dermatology
关键词
佩吉特病
乳腺外
黏蛋白类
基因表达
Paget＇s disease, extramammary
Mucins
Gene expression
