摘要
目的:研究不同胆囊疾病中E钙黏蛋白(E-cad)、血管内皮生长因子(VEGF)及增殖细胞核抗原(PCNA)的表达和分布的差异,探讨E-cad、VEGF和PCNA表达与胆囊癌临床病理特征的相关性及其预后判断的价值。方法:收集胆囊癌标本41例,胆囊腺瘤标本14例,慢性胆囊炎10例,应用免疫组化染色SP法分析。结果:胆囊癌VEGF阳性表达率(33/41)显著高于胆囊腺瘤(6/14)和胆囊炎(0/10),F=24·831,P=0·000,而E-cad阳性表达率(18/41)明显降低,伴有转移者E-cad表达(12/30)更低;胆囊癌PCNA LI高于胆囊腺瘤和胆囊炎组,H=19·03,P=0·008。VEGF过度表达者3年生存率(6/25)较阴性组(5/6)显著降低,F=8·286,P=0·008,PC-NA LI≥50者3年生存率(1/16)较<50者(10/15)显著降低,F=16·151,P=0·000;E-cad高表达者3年生存率(9/16)较阴性组(1/15)明显增高,F=12·511,P=0·001。结论:E-cad和VEGF及PCNA表达在胆囊癌、胆囊腺瘤与慢性胆囊炎之间差异均有统计学意义,均可作为评估胆囊良、恶性肿瘤的参考指标和胆囊癌预后判断的参考指标。
OBJECTIVE：To study the patterns of the expressions of E-cad, VEGF and PCNA in gallbladder carcinoma,adenoma and chronic choleeystitis, and explore the correlation of E-cad, VEGF and PCNA expressions with gallbladder carcinoma. METHODS： Studies were performed in 41 samples of gallbladder carcinoma, 14 cases of gallbladder adenoma and 10 cases of chronic choleeystitis with immunohistochemistry. RESULTS：The positive rate of VEGF （33/41） in gallbladder carcinoma was significantly higher than rates of gallbladder adenomas and chronic cholecystitis, F=24. 831 ,P=0. 000, the PCNA LI in gallbladder carcinoma was significantly hingher than rates of gallbledder adenomas and chronic choleeystitis, H=19. 030, P=0. 008. The positive rate of E-cad （18/41）in gallbladder carcinoma was significant lower than rates of gallbladder adenomas and eholeeystitis, F=8. 286, P=0. 008. Three-year survival rate was significantly lower in gallbladder carcinoma with VEGF（6/25） and PCNA （1/16）over expression, F=16. 151,P= 0. 000, and three-year survival rate in the patients with E-cad positive tumors （9/16） was higher than that of those with negative tumors （1/15）, F = 12. 511, P =0. 001. CONSLUSIONS： The expression of E- cad, VEGF and PCNA are significantly different among gallbladder carcinoma, adenoma and chronic cholecystitis. Therefore, benign or malignant gallbladder tumor may be distinguished by detecting the expressions of E-cad, VEGF and PCNA, and they would be valuable for predicting prognosis of gallbladder carcinomas.
出处
《中华肿瘤防治杂志》
CAS
2006年第7期508-511,共4页
Chinese Journal of Cancer Prevention and Treatment
