摘要
为了解Vc的免疫机制,以中国对虾为实验动物,对其投喂添加不同Vc含量(0%、0.5%、1%、2%)的饲料,进行存活率、iNOS、CAT、LZM酶活性及TLR、NF-κB表达水平的比较。结果发现:(1)饲喂Vc的不同处理组提高了中国对虾的存活率、iNOS、CAT及LZM活性,其中1%添加组存活率达90.28%,显著高于其他组。(2)Vc可以调节TLR、NF-κB在血清及鳃中的表达水平。1%添加组血清、鳃中TLR的表达水平相对于各自的对照组表现出显著性差异(P<0.05)。TLR、NF-κB在组织中的表达趋势并不一致。1%组血清中NF-κB的表达水平高于对照组,其他两组的表达水平始终位于对照组之下,而2%添加组鳃中的表达水平高于其他三组,说明Vc可以调节这两个免疫基因的表达,在中国对虾的免疫应答中发挥重要作用。
In order to deeply understand the immune function of Vc,we fed different levels（0%,0.5%,1%,2%）of Vc to study the influence on the Chinese shrimp.We detected survival rate and activity of iNOS,CAT and LZM at the different experiment times of Chinese shrimp.Using real-time fluorescent quantitative RT-PCR approach,expression TLRs and NF-κB genes were examined in serum and gills of the Chinese shrimp.Results showed that：（1） VC can raise the survival rate and the activity of iNOS,CAT and LZM.The 1% groups show the best immune activating effects among groups,with survival rate at 90.3%,significantly higher（P〈0.05）than that of other groups.（2） Vc has regulatory activity on mRNA expression of TLR and NF-κB.There are significant differences（P〈0.05）on TLR expression in the serum and gill of 1% group compared to the control.NF-κB mRNA expression in serum of 1% group is higher than that in other groups,but that in 2% group has the highest levels in early six days,and then declines even lower than the control.NF-κB mRNA expression in gill of 2% group is higher than control.Overall,these results demonstrate that Vc can effectively improve the survival rate and the activity of three non-specific immunity enzymes of the Chinese shrimp and regulate the expression level of TLRs and NF-κB.
出处
《水产学报》
CAS
CSCD
北大核心
2011年第2期200-207,共8页
Journal of Fisheries of China
基金
国家虾产业技术体系(nycytx-46)
公益性行业专项(200803012)
公益性农业行业科研专项(201103034)
