摘要
以凡纳滨对虾Litopenaeus vannamei为对象,投喂添加不同水平的复方中草药饲料,研究中草药对凡纳滨对虾生长性能和血清非特异免疫因子的影响。试验共分7组,A^F组饲料中分别添加质量分数为0.5%、0.9%、1.3%、1.7%、2.1%、2.5%的复方中草药,对照组饲料中不添加。饲养试验共进行56 d。结果表明:随着复方中草药添加水平的提高,凡纳滨对虾的增重率和特定生长率逐渐增加,仅F组与对照组差异显著(P<0.05);饲料系数和成活率各组间均无显著差异(P>0.05);增重率与复方中草药添加水平的回归方程为Y=-125.86X2+521.93X+6063(R2=0.9561)。由方程可知:当复方中草药水平为2.07%时增重率最高;对虾血清中超氧化物歧化酶、碱性磷酸酶、酚氧化物酶和溶菌酶随着复方中草药添加水平的增加表现出先显著增加然后下降的趋势,E组中4种酶活力均最高;复方中草药对酸性磷酸酶和过氧化物酶活力没有显著影响。
A feeding experiment was conducted to evaluate the effects of different supplemental levels of dietary Chinese herbal mixture of Astragalus, Isatis root, Honeysuckle and Gypsum with an equal ratio on growth and nonspecific immunity in Pacific white shrimp Litopenaeus vannamei. The shrimp juveniles with an average body weight of 0.1 g were stocked into a culture system and fed the diet containing 41% of crude protein supplemented with the Chinese herbal medicine at a rate of 0.0%, 0.5%, 0.9%, 1.3%, 1.7%, 2.1% and 2.5% for 56 days. Weight gain rate（WGR） and specific growth rate of the shrimp were found to increase with the increase in the dietary Chinese herbal mixture, but to increase significantly only in the 〈0.05）. There were no significant differences among all Through quadratic model regression analysis between WGR group of 2.5 % level compared to the control group （P treatments for feed conversion ratio and survival rate. and the Chinese herbal compound levels in the diets,one regression equation was expressed as Y = - 125. 86X^2 ＋ 521.93 ＋ 6063 （R^2 = 0.9561 ）. The maximal WGR was observed when the Chinese herbal compound level in diets was 2.07%. The activities of superoxide dismutase, phenoloxidase, lysozyme and alkaline phosphatase were significantly higher in the serum of the shrimp fed test diets than that in the control. However, the activities of serum acid phosphatase and peroxidase were not significantly influenced by the dietary Chinese herbal levels, indicating that the Chinese herbal compound supplementation improves the growth, feed utilization and the ability of non- specific immunity in the shrimp.
出处
《大连水产学院学报》
CAS
CSCD
北大核心
2009年第2期162-165,共4页
Journal of Dalian Fisheries University
基金
国家科技支撑计划项目(2006BAD03B03)
福建省青年创新基金资助项目(2005J036)
