摘要
目的:建立大鼠心肌梗死后蛋白表达变化谱,以进一步了解心肌梗死后心肌细胞重塑产生机制。方法:通过结扎大鼠冠状动脉左前降支建立急性心肌梗死模型,利用蛋白质双向凝胶电泳技术(two-dimensionalgelelectrophoresis,2-DE)分离心肌总蛋白,采用PDQuest7.3.1软件比较分析,获得差异表达蛋白总体变化趋势。进一步通过蛋白免疫印记技术(Western-blotting)检测碱性成纤维生长因子(Basefibroblastgrowthfactor,bFGF)在心肌梗死后的表达变化。结果:成功建立了大鼠急性心肌梗死模型;2-DE结果表明:以假结扎组为对照,梗死3天组有27个蛋白显著上调,18个蛋白显著下调,7个蛋白表达明显差异(ratio>5)。进一步研究发现梗死区心肌组织bFGF表达明显升高。结论:心肌梗死后蛋白表达变化趋势的探讨为心室重塑机制研究提供线索。
Objective：To investigate the mechanism of ventricle remodeling after myocardial infarction by analyzing the proteins detected by ampholyte pH gradients based two-dimensional gel electrophoresis（2-DE）.Methods：The animal model of acute myocardial infarction was established by surgical legation of the left coronary artery in rat.The total proteins of normal and infracted cardiac muscle tissues were extracted.The differentially expression proteins were selected by using image analysis software.The expression of bFGF were analyzed by western-blotting.Results：The rat model of acute myocardial infarction was established and identified in ligation 3 days group.There were 27 up-regulated and 18 down-regulated,while 7 proteins specially expressed in infracted tissue were obviously changed.Conclusion：Myocardial infarction caused differentially expression change for cardiac muscle tissues,which were helpful to study the metabolism of ventricle remodeling.
出处
《现代生物医学进展》
CAS
2011年第2期230-232,共3页
Progress in Modern Biomedicine
关键词
心肌梗死
双向电泳
蛋白印记
碱性成纤维生长因子
Myocardial infarction
Two-dimensional gel electrophoresis（2-DE）
Western-blotting
Base fibroblast growth factor
