摘要
目的探讨2000μW／cm^2电磁辐射对大鼠海马N-甲基-D-门冬氨酸（NMDA）受体NRI亚单位蛋白及其mRNA水平表达的影响，揭示电磁辐射对大鼠学习记忆功能的损伤机制。方法实验分为空白对照组，假辐射组，1h／d、2h／d、3k／d辐射组。将辐射组大鼠固定体位，头部接受功率密度为2000μW／cm^2的近场辐射，连续辐射30d。通过Mogis水迷宫检测大鼠的空间学习记忆能力，采用免疫组化法和Western—Blot法检测大鼠海马组织NRl蛋白表达的变化，RT—PCR法检测大鼠海马组织NRlmRNA表达的变化。结果各辐射组大鼠在水迷宫检测第4天寻找安全平台的逃避潜伏期分别为1h／d[（12．29±1．36）S]、2h／d[（17．99±2．25）S]、3h／d[（24．66±5．56）S]，均明显长于空白对照组[（8．8±1．66）S]（尸〈0．05）；1h／d、2h／d和3h／d辐射组大鼠海马神经元均排列紊乱，NRl阳性细胞比率明显低于空白对照组，海马组织NR1蛋白[分别为（0．122±0．026）、（0．102±0．023）、（0．060±0．009）]及其mRNA[分别为（0．46±0．07）、（0．35±0．05）、（0．12±0．02）]表达水平较空白对照组[（10．70±0．11）、（0．68±0．11）]均明显降低（P〈0．05）。而假辐射组大鼠各项指标与空白对照组相比均无显著差异（P〉0．05）。结论2000μW／cm^2电磁辐射可导致大鼠学习记忆功能下降，其机制可能与大鼠海马组织NR1蛋白及其mRNA的表达降低有关．
Objective To evaluate the effects of electromagnetic irradiation of 2000 μW/cm2 exposure on mRNA and protein expression levels of immunoreactive protein and mRNA of NMDAR1 in rats hippocampal, and to explore the impaired mechanism of electromagnetic irradiation on learning and memory. Methods Rats were randomly divided into normal control group, sham-radiated group, and 1 h/d,2 h/d, and 3 h/d radiation uroups. The rats in the radiation groups were fixed and recieved microwave exposure of 2000 μW/cm2 , then their learning and memory abilities were tested by Morris water maze experiment, the change of NR1 protein in hippocampal neurons of each group of rats was measured with immunotlistoehmistry and western blot techniques, and the expression of NR1 mRNA in hippocampus was determined by RT-PCR. Results In the water maze test,compared with the normal control group（8.8 ± 1.66） ,the escape lateney of three radiated groups rats （ 1 h/d （ 12.29± 1.36 ） s,2 h/d （ 17.99 ± 2.25 ） s, and 3 h/d （24.66 ± 5.56） s） were significantly longer （P 〈 0.05 ）. In the radiation group,the hippocampal neurons of rats showed evident reduction in the ratio of NR1 positive cells,irregu- lar,and arrayed in disorder. Moreover, compared with the normal control group （ （0.70 ± 0. 11 ）, （0.68 ± 0. 11 ） ）, the expession of NR1 protein （ 1 h/d （0. 122 ±0. 026） ,2 h/d （0. 102 ＋ 0. 023 ） , and 3 h/d （0. 060 ± 0. 009） ） and itsmRNA （1 h/d （0.46 ±0.07） ,2 h/d （0.35±0.05） ,and3 h/d （0.12±0.02）） inhippocampal neurons was significantly decreased （P 〈 0.05）. Among the indicators,there was no significant difference between sham-radiated group and normal control group. Conclusions Electromagnetic irradiation of 2000 μW/cm2 exposure can impair the learning and memory abilities of rats possibly through a mechanism correlated with the low- er expression of NR1 protein and its mRNA in hippocampus.
出处
《中华行为医学与脑科学杂志》
CAS
CSCD
北大核心
2011年第2期111-114,共4页
Chinese Journal of Behavioral Medicine and Brain Science
基金
基金项目：河北省自然科学基金资助项目（C2007000921）
河北省卫生厅资助项目（20100136）
关键词
电磁辐射
海马
学习记忆
NR1亚单位
Electromagnetic radiation
Hippocampus
Learning and memory
NR1 subunit
