摘要
目的建立高效液相色谱法测定棉油皂脚中总棉酚(TGP)与游离棉酚(FGP)含量的方法。方法采用WatersBondapak C18 Column(3.9 mm×150 mm,4μm);流动相为乙腈-0.7%磷酸水溶液(80∶20);流速为1.0 mL.min-1;检测波长为238 nm;柱温为25℃。结果棉酚在10～60μg.mL-1(r=0.999 4)内线性关系良好,平均加样回收率为99.03%,RSD=1.72%(n=3)。结论本法简便、快速、准确、重复性高,可用于对棉油皂脚中棉酚的含量测定。
OBJECTIVE To determine the content of total gossypol and liberation gossypol by HPLC.METHODS The analysis was performed on a Waters Bondapak C18 Column（3.9 mm×150 mm,4 μm） at 25 ℃with acetonitrile-0.7% phosphoric acid water solution（80∶20） as the mobile phase and the flow rate of 1.0 mL·min.1.The detection wavelength was set at 238 nm.RESULTS Gossypol have good linearity in the range of 10.60 μg·mL.1（r=0.999 4）.The average recovery was 99.03%,RSD=1.72%（n=3）.CONCLUSION The developed method is convenient,rapid and accurate with high repeatability,which is suitable to assay the quality of gossypol.
出处
《中国现代应用药学》
CAS
CSCD
北大核心
2011年第2期162-164,共3页
Chinese Journal of Modern Applied Pharmacy
基金
新疆建设兵团博士基金项目(2009JC14)
国家教育部科学技术研究重点项目(205185)
