摘要
目的:探讨肝细胞生长因子(HGF)和血管内皮生长因子(VEGF)在喉鳞状细胞癌早期诊断、发生发展中的作用及其与临床病理特征及淋巴结转移的关系。方法:应用酶联免疫吸附法测定54例喉鳞状细胞癌患者血清VEGF和HGF水平在术前及术后1、3、7、14d的变化,并测定30例声带息肉组及35例对照组血清VEGF和HGF水平。结果:①声带息肉组和对照组血清中都有微量的VEGF和HGF,喉癌组术前血清VEGF和HGF水平均显著高于声带息肉组和对照组(P<0.01),声带息肉组与对照组差异无统计学意义(P>0.05);②喉鳞状细胞癌患者术前血清VEGF和HGF水平与其临床分期、组织学分级和淋巴结转移有关(P<0.01),而与肿瘤部位、年龄和性别无关(P>0.05);③喉鳞状细胞癌患者血清VEGF与HGF呈显著正相关(r=0.7667,P<0.01);④喉鳞状细胞癌患者术后血清HGF含量明显升高,术后3d达高峰,术后7、14d逐渐下降并且明显低于术前水平;VEGF水平术后逐渐下降。结论:VEGF和HGF在喉鳞状细胞癌的发生中具有重要作用,与喉鳞状细胞癌的发生和发展有关,血清VEGF和HGF水平增高在喉鳞状细胞癌中预示了一种更具有侵袭性的生物学行为。
Objective：To investigate the role and clinical value of the serum level of hepatocyte growth factor（HGF） and vascular endothelial growth factor（VEGF） in laryngeal squamous cell cancer and the relationship between HGF,VEGF and clinicopathological factors.Method：We measured serum HGF and VEGF level in 54 laryngeal squamous cell cancer patients using ELISA to demonstrate the variation of HGF,VEGF level before operation and at day 1,3,7,14 post-surgery.The concentration of the serum HGF,VEGF content in 35 normal healthy people and in 30 vocal cords polyps patients were measured by the quantitative sandwich enzyme linked immunoassay technique.Result：①The level of serum HGF and VEGF in the patients with laryngeal squamous cell cancer was significantly higher than that of normal healthy people and vocal cord polyps patients（P0.01）.②The level of serum HGF,VEGF in the patients with laryngeal quamous cell cancer was related to clinical stage,differentiation situation,lymph nodes metastasis（P0.01）,but it was independent of age at diagnosis,gender and tumor location（P0.05）.③There was a significantly positive correlation between preoperative serum VEGF and HGF levels（r=0.766 7,P0.01）.④Post-operational serum HGF levels in 54 laryngeal squamous cell cancer patients who underwent surgical intervention increased significantly,peaked at day 3 after operation.Serum HGF levels of survivors during follow-up period gradually decreased at day 7 and day 14 after operation.The postoperative serum levels of VEGF were decreased significantly than that of preoperation.Conclusion：HGF and VEGF may play an important role in the development and progression of human laryngeal cancer.Elevated serum HGF and VEGF levels predict a more aggressive biological behavior in laryngeal squamous cell cancer.
出处
《临床耳鼻咽喉头颈外科杂志》
CAS
CSCD
北大核心
2010年第24期1112-1115,共4页
Journal of Clinical Otorhinolaryngology Head And Neck Surgery
关键词
喉肿瘤
肝细胞生长因子
血管内皮生长因子
血清
laryngeal neoplasms
hepatocyte growth factor
vascular endothelial growth factor
serum
