摘要
实验确定了新鲜灵芝孢子进行低能N+离子注入的适宜参数:注入剂量1.25×1016ions.cm-2,注入能量20 KeV,靶室真空度10-3Pa,以5 s脉冲式注入,间隔为15 s。离子注入后,筛选酯酶同工酶酶谱存在显著差异和拮抗实验呈阳性的突变菌株,分别以液体发酵菌丝体多糖含量、固体栽培子实体生物转化率为初筛和复筛标准,经遗传稳定性实验验证,筛选到了一株灵芝高产菌株GL1026,其灵芝子实体产量、多糖含量、三萜含量分别较对照菌株提高了15.94%、9.68%、17.60%。结果表明:低能N+离子束注入是一种较为理想的灵芝育种手段。
Low energy N＋ion beam implantation was used in the breeding of Ganoderma lucidum in our research.The optimum operation condition and some controlling parameters of the implantation using fresh spores can be determined as follows： the implantation dose is 1.25×1016ions·cm-2,the energy is 20KeV,the vaccum is 10-3Pa,and a 5-second pluse with a 15-second internal.The mutation strains were determined by EST isoenzyme analysis together with the antagonistic experiment.Superior strains were selected according to the polysacchrides rate of liquid fermentation and biological efficiency of solid culturavation.After the hereditary stability experiment,a high yielding strain was got.The study indicated the practicality of the ion beam implantation method in the breeding of Ganoderma lucidum.
出处
《药物生物技术》
CAS
CSCD
2011年第1期32-37,共6页
Pharmaceutical Biotechnology
基金
江苏省科技支撑计划--农业部分(项目编号:BE2009308-2)
