摘要
【目的】建立测定红景天药材根茎中红景天苷含量的HPLC方法,为进一步研究红景天的药材质量提供依据。【方法】采用Thermo C18柱（250 mm×4.6 mm,5μm）;乙腈-水-四氢呋喃（5.5：93：0.4）为流动相;流速为1.0 ml/min;紫外检测波长为270 nm;柱温30℃。【结果】红景天苷在2.5-40μg/ml范围内与峰面积呈良好的线性关系,回归方程为Y=1×10^7X-2428,r=0.9996。平均回收率为96.45%,RSD为0.63%（n=9）。【结论】本法灵敏,准确,稳定性与重现性均良好,可用于红景天药材中红景天苷的含量测定。
【Objective】To determine the content of salidroside in Rhodiola kirilowii（Regel） Maxim.by HPLC.【Methods】HPLC analysis was carried out on Thermo C18 column（250 mm×4.6 mm,5μm）.The mixture of acetonitrile-THF-water（5.5：93：0.4） was used as the mobile phase.The flow rate was 1.0 ml/min.The detection wavelength was at 270 nm.The column temperature was at 30 ℃.【Results】The linear range of salidroside was 2.5~40 μg/ml,the recovery of this method was 104.05% and the RSD was 0.54%（n =5）.【Conclusion】The method was sensitive,accurate and reproducible,and to suitable for the quality control of Rhodiola kirilowii（Regel） Maxim.
出处
《武警医学院学报》
CAS
2011年第3期173-174,178,共3页
Acta Academiae Medicinae CPAPF
基金
国家自然科学基金资助项目(30672774
81073152)
天津市科委重点项目(10JCZDJC21100)
关键词
狭叶红景天药材
红景天苷
高效液相色谱法
Rhodiola kirilowii（Regel） Maxim
Salidroside
High performance liquid chromatography
