摘要
目的:研究狭叶红景天的化学成分;评价狭叶红景天提取物及其化学成分体外抗结核分枝杆菌(ATCC 27294)活性。方法:运用硅胶,Sephadex LH-20,RP-18等柱色谱及半制备液相色谱对狭叶红景天的化学成分进行分离纯化,并利用核磁共振、质谱等技术,鉴定它们的化学结构;通过测试体外最低抑制浓度(minimal in- hibitory concentrations,MIC)和最低杀菌浓度(minimal bactericidal concentrations,MBC)来评价有关物质的抗结核-分枝杆菌活性。结果:分离、纯化并鉴定了12个化合物,分别为β-谷甾醇(β-sitosterol,1),酪醇(tyrosol,2),反式-对羟基肉桂酸(trans-4-hydroxycinnamic acid,3),香叶醇β-吡喃葡萄糖苷(geranyl-β-glucopyranoside,4),橙花醇β-吡喃葡萄糖苷(neryl-β-glucopyranoside,5),正己醇β-吡喃葡萄糖苷(hexylβ-glucopyranoside,6),没食子酸(gallic acid,7),表没食子儿茶素-3-没食子酸酯[(-)-epigallocatechin gallate,8],棉皮素7-O-α-吡喃鼠李糖苷(rhodiolgin,9),异落叶松树脂醇9-O-β-吡喃葡萄糖苷(isolariciresinol-9-O-β-glucopyranoside,10),正辛醇α-吡喃阿拉伯糖(1→6)-β-吡喃葡萄糖苷(rhodiooctanoside,11),和橙花醇α-吡喃阿拉伯糖(1→6)-β-吡喃葡萄糖苷(sacranoside B,12)。结论:化合物3,6,9～12为首次从狭叶红景天中发现,化合物4,5为首次从红景天属植物中分得;狭叶红景天80%乙醇提取物、醋酸乙酯萃取物以及化合物7,8,对结核分枝杆菌具有一定抑制和杀灭活性。
The chemical constituents of Rhodiola kirilowii were separated and purified by repeated column chromatography on silica gel, RP - 18, Sephadex LH -20 and semi-preparative HPLC. Each compound was characterized by spectroscopic and physical data. Twelve compounds have been purified and identified to be β-sitosterol （ 1 ）, tyrosol （2）, trans-hydroxycinnamic acid （3）, gera- nyl β-glucopyranoside （4）, neryl β-glucopyranoside （5）, hexyl β-glucopyranoside （6）, gallic acid （7）, （ - ）-epigallocatechin gallate （8）, rhodiolgin （9）, isolariciresinol-9-O-β-glucopyranoside （10）, rhodiooctanoside （ 11 ）, and sacranoside S （ 12 ）. Among them, compounds 3, 6, 9-12 were isolated from Rhodiola kirilowii for the first time ; Compounds 4 and 5 were obtained for the first time from the genus Rhodiola. The in vitro activities against Macobacterium tuberculosis （ATCC 27294） of its extracts and pure components were evaluated by testing their MIC （ minimal inhibitory concentration） and MBC （ minimal bactericidal concentration）. The 80% （ a. q. ） EtOH extract, EtOAc-soluble extract, compounds 7 and 8 exhibited in-vitro inhibitory and bactericidal activities against Macobacterium tuberculosis in different extent.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2008年第13期1561-1565,共5页
China Journal of Chinese Materia Medica
基金
香港研究资助局(RGC)课题(CUHK4316/03M)
