摘要
为研究桃小食心虫Carposina niponensis Walsingham自然种群过冷却能力的变化动态,从生理生化水平上探讨桃小食心虫幼虫耐寒机制,测定了桃小食心虫幼虫在越冬前后不同月份的过冷却点、体内含水量、脂肪、蛋白和糖原的含量。结果表明:桃小食心虫越冬幼虫的过冷却点(super-cooling point,SCP)和结冰点(freezing point,FP)随越冬期温度降低而逐渐降低,并在冬季过后随温度升高而逐渐升高,其中在3月份时最低,分别为-14.89℃和-9.95℃,显著低于其它月份。幼虫体内含水量、总蛋白含量、糖原含量在越冬前后变化趋势与SCP变化相似并且各自又有不同的特点,但在2月份时都达最低,分别为44.83%、32.44μg/mg、1.95μg/mg。幼虫体内的总脂肪含量由越冬初期(2008-10)的29.04%逐渐降低至越冬后期(2009-06)的15.56%。结果说明桃小食心虫幼虫越冬过程中体内水分、总蛋白、糖原等生化物质含量的变化与其抗寒能力存在一定的联系,显示了其对冬季温度变化的生态适应。
The peach fruit moth,Carposina niponensis Walsingham,is one of the key insect pests on fruit trees,such as apple,peach and hawkthorn.Its damages have been growing up in recent years because of the effects of fruit cultivation model,the adjustment of planting structure and global warming.Under natural conditions the moth develops one to two generations in Shandong province and overwinters as cocooned larvae for nearly eight months in the upper layer of soil.In the present study,experiments were carried out to examine the larval super-cooling capacity,the content of water,fat,protein and glycogen in the body during the overwintering period.Results showed that the super-cooling ability of larvae was gradually strengthened with the dropping of winter temperature,and weakened with the increasing of the temperature after winter.The super-cooling point（SCP） and freezing point（FP） of the larvae in March dropped to the lowest,-14.89℃ and-9.95℃,respectively,significantly lower than those in other months,while there was no significant difference between the pre-and late-overwintering months.The dynamics of water,protein and glycogen content in the body were similar to that of SCP although they had their own characters during overwintering.The content of water,protein and glycogen reached to the lowest in February,44.83%,32.44μg/mg and 1.95μg/mg,respectively.The fat content in the body decreased from 29.04% in October to 15.56% in June during the overwintering.The fat and glycogen content in pre-overwintering months were remarkably higher than those in mid-and late-overwintering months.Though there is no statistically significant correlation between the larval SCP and the content of total fat,total protein,glycogen or water in the body,their similar changing trends during the overwintering still indicate a quite close relationship between the larval SCP and the total content of the three biochemical substances or water content.Our study reflects that the overwintering larvae of C.niponensi can adjust their beha
出处
《生态学报》
CAS
CSCD
北大核心
2011年第3期638-645,共8页
Acta Ecologica Sinica
基金
农业公益性行业科研专项(2000803006)
山东省利用全球环境基金赠款项目(TF090802)
关键词
桃小食心虫
过冷却点
含水量
脂肪含量
蛋白含量
糖原含量
Carposina niponensis
super-cooling point
water content
fat content
protein content
glycogen content
