摘要
目的研究淡色库蚊越冬蚊体内海藻糖和海藻糖酶含量的动态变化。方法利用ELISA法测定不同越冬时期淡色库蚊雌成蚊体内海藻糖和海藻糖酶的含量。结果越冬期淡色库蚊滞育蚊所占百分比先增加后减少,越冬中期淡色库蚊全部为滞育蚊。淡色库蚊体内海藻糖含量先增加后降低,到1月份时达最大值,为(225.77±7.461)ng/ml,3月份降至最小值(132.41±2.37)ng/ml。不同越冬时期越冬蚊体内海藻糖含量差异有统计学意义(F=53.59,df=150,P<0.05);海藻糖酶含量先降低后增加,至1月份达最大值,为(9.32±0.23)ng/ml,到越冬末期含量持续减少。不同越冬期淡色库蚊体内海藻糖酶含量差异有统计学意义(F=84.21,df=150,P<0.05)。结论越冬淡色库蚊体内海藻糖和海藻糖酶含量变化较大,在气温最低的1月份含量达最大值,由此推断,海藻糖和海藻糖酶在淡色库蚊越冬过程中起重要作用。
Objective To ascertain dynamic changes in trehalose and trehalase levels in Culex pipiens pallens.Meth-ods Trehalose and trehalase levels in female adult mosquitoes at different overwintering periods were detected using an enzyme-linked immunosorbent assay.Results As overwintering time went on,the percentage of diapausing mosquitoes was increased at first and then decreased,and in mid-overwintering,all mosquitoes were found to be in a state of diapause.The trehalose level in adults increased at first and then decreased;it peaked in January (225.77±7.461ng/ml) and reached its trough in March (132.41±2.37ng/ml).The trehalose level in overwintering mosquitoes differed significantly during different overwintering periods (F=53.59,d f=95,P=0.00).The trehalase level decreased at first and then increased;it peaked in January (9.32±0.23ng/ml)and continued to decrease at the end of overwintering.The trehalase level in mosquitoes differed significantly during different overwintering periods (F=84.21,df=95,P=0.000). Conclusion Trehalose and trehalase levels in overwintering mosquitoes vary greatly and peak in January when the temperature is lowest.Therefore,trehalose and trehalase play important roles in overwintering mosquitoes.
作者
张本光
臧爱梅
王利磊
程鹏
郭秀霞
王海防
刘丽娟
ZHANG Ben-guang;ZANG Ai-i-mei;WANG Li-lei;CHENG Peng;GUO Xiu-xia;WANG Hai-fang;LIU Li-juan(Shandong Academy of Medical Sciences,Shandong Institute of Parasitic Diseases,Jining,Shandong,China 272033;Gaomi County Bureau of Agriculture,Gaomi,Shandong,China 261500)
出处
《中国病原生物学杂志》
CSCD
北大核心
2018年第12期1335-1337,1343,共4页
Journal of Pathogen Biology
基金
山东省自然科学基金(ZR2017YL005)
山东省医学科学院医药卫生科技创新工程项目
山东省医科院发展计划(No.2017-05).
关键词
淡色库蚊
海藻糖
海藻糖酶
蚊媒传染病
Culex pipiens pallens Coquillett
trehalose
trehalase
mosquito-borne infectious diseases
