摘要
醇溶蛋白是谷子籽粒主要的贮藏蛋白,研究谷子醇溶蛋白的差异对于谷子品种选育及种质鉴定评价有重要的意义。本研究利用SDS-PAGE方法对来自不同省份的46份谷子材料进行醇溶蛋白谱带分析,结果表明,单个品种可分离出14~24条醇溶蛋白谱带,平均每个品种18.74条带,46份品种共分离出32条醇溶蛋白谱带,其中7条为共有带,25条为多态性带,多态性带数占分离出总带数的78.1%。根据迁移率大小将醇溶蛋白谱带分为A、B、C、D四个区段,四个区段内分别有6、16、15、4种带型。在B、C区段醇溶蛋白有较丰富的多态性,A、B区段弱带较多,C、D区段强带较多。醇溶蛋白分子量在13.0~55.0 kDa之间,在29,25 kDa（第23条带）、22.5 kDa（第26条带）、17.5 kDa（第28条带）位点附近醇溶蛋白表达丰富且品种之间存在较大差异。品种之间醇溶蛋白谱带存在较大的差异,可作为品种鉴定与评价的重要依据。聚类分析表明,在GD值为0.45时,46个品种可以分为5类,其中34个品种聚为一类,其他四类所包含的材料较少,这表明大部分材料醇溶蛋白相似度较高。
Prolamin is the main storage protein of foxmail millet,and it is meaningful for the research of prolamin polymorphism in millet breeding and genetic resource evaluation.With SDS-PAGE,prolamin polymorphism of 46 foxtail varieties from different province was analyzed.The results indicated that 14-24 prolamin bandings could be identified from one variety,and each variety contained 18.74 bands on average.There were 32 bands in 46 varieties,25 of them showed polymorphism（amount to 78.1%） and 7 of them were shared by all the varieties.Prolamin bands could be divided into A,B,C and D sections based on mobile rate,and there were 6,16,15,4 band patterns in four sections,respectively.Ppolymorphism is rich in B and C section.Bands in A and B sections were relatively weaker,and that stronger in C and D sections.The molecular weight of prolamin was 13.0-55.0 kDa.The prolamin bands around the 29,25 kDa（No.23）,22.5 kDa（No.26）,17.5 kDa（No.28） sites,were relatively stronger,and significantly different among varieties.The research result could be used in variety identification and evaluation.The cluster analysis of prolamin indicated that 46 varieties fallen into five categories based on the GD value at 0.45,in which 34 varieties were in the same categories and the other four categories only contained a few varieties.The results indicated that most of the materials were of high similarity.
出处
《华北农学报》
CSCD
北大核心
2010年第6期87-91,共5页
Acta Agriculturae Boreali-Sinica
基金
现代农业产业技术体系建设专项资金
关键词
谷子
籽粒醇溶蛋白
遗传差异
Foxtail millet（Setaria italica L.Beauv.）
Seed prolamin
Genetic diversity
