摘要
目的:利用发根农杆菌诱导绞股蓝,得到毛状根。方法:采用外植体共感染接种法诱导绞股蓝毛状根,研究了不同菌株、外植体类型、预(共)培养时间、菌液浓度、浸染时间、乙酰丁香酮(As)和除菌培养基类型等因素对转化率的影响。结果:利用A4菌浸染不经过预培养的叶片,当发根农杆菌浓度在OD 600值为0.8,浸染10 min,共培养2 d,100μmol/L As、MS+300 mg/L Cab为除菌培养基为绞股蓝毛状根诱导的最佳条件。结论:用外植体共感染法诱导出绞股蓝毛状根,并确定了最佳诱导条件。
Objective：To induce hairy roots of Gynostemm apentaphyllum by Agrobacterium rhizogenes strains.Methods： Hairy roots were induced by the co-culture method of explants and Agrobacterium rhizogenes strains.Effects of different Agrobacterium rhizogenes strains,explants,pre（co）-culture time,Bacterial concentration,infecting time,As concentration and antibiotic medium on the transformation frequency were studied.Results： The highest induction frequency was obtained form leaf 2 days co-cultivation,which were induced by Agrobacterium rhizogenes OD 600 0.8 for 10 min,100 μmol/L As and MS＋300 mg/L Cab.Conclusion： Hairy roots were induced by co-cultivation and the optimum induced condition were determined.
出处
《中药材》
CAS
CSCD
北大核心
2010年第11期1675-1677,共3页
Journal of Chinese Medicinal Materials
关键词
绞股蓝
毛状根
诱导
Gynostemm apentaphyllum（Thunb） Makino
Hairy roots
Induction
