摘要
目的:观察硫化氢对帕金森病(Parkinson’s disease,PD)大鼠神经行为学、氧化应激以及多巴胺及其代谢产物的影响。方法:采用6-羟基多巴胺(6-OHDA)注射于脑右侧黑质造成偏侧PD模型。将模型动物随机分为模型组、硫化氢组(硫氢化钠做供体),每组10只;另分别取10只正常大鼠为正常组及假手术组(以抗坏血酸注射)。观察PD大鼠经过硫化氢处理后神经行为学、脑组织丙二醛(MDA)、谷胱甘肽(GSH)、超氧化物歧化酶(SOD)含量以及多巴胺代谢产物的变化。结果:与模型组比较硫化氢组大鼠行为学明显改善(P<0.01),同时脑组织中GSH、SOD含量均升高,MDA含量降低,多巴胺及其代谢产物升高。结论:硫化氢对PD模型大鼠多巴胺神经元具有保护作用。
Objective：To observe the effects of H2S prescription on neuroethology,oxidative stress,dopamine and its metabolites in rats with Parkinson＇s disease（PD）.Methods： Model of hemilateral PD rat was established with injection of 6-hydroxydopamine（6-OHDA）into the right substantia nigra of midbrain.The PD model rats were randomly divided into model group,H2S group（sodium hydrosulfide,NaHS,was used as H2S donor）,control group and sham-operated group（injected by ascorbic acid）,10 rats for each group.The changes of neuroethology,the contents of malondialdehyde（MDA）,glutathione（GSH）,superoxide dismutase（SOD）,and the levels of dopamine and its metabolites were compared among groups.Results：Compared with model group,the neuroethology of rats in H2S group were significantly improved.The contents of GSH,SOD were reduced significantly;The contents of MDA and the levels of dopamine（DA）,HVA,dihydroxv-phenyl acetic acid（DOPAC） were elevated significantly.Conclusion： H2S can protect the dopamine neurons in rats with Parkinson＇s disease.
出处
《神经解剖学杂志》
CAS
CSCD
北大核心
2011年第1期59-62,共4页
Chinese Journal of Neuroanatomy
基金
湖南省衡阳市科技局课题(2009HKJ12)
