摘要
目的探讨新生儿败血症的病原菌分布及耐药性,以合理地选择抗菌药物。方法采用先德VersaTREK-240全自动血培养仪培养血样本,并以ARIS 2X微生物分析系统进行细菌鉴定及药敏分析。结果 890份血培养分离出病原菌159株,其中革兰阳性球菌占94.97%,革兰阴性杆菌占4.40%,真菌占0.63%;以凝固酶阴性葡萄球菌为主,其次为金黄色葡萄球菌、链球菌属;葡萄球菌属对青霉素、氨苄西林、苯唑西林、红霉素等耐药率高;革兰阳性球菌中未发现对万古霉素耐药的菌株,耐甲氧西林葡萄球菌检出率在表皮葡萄球菌、金黄色葡萄球菌、溶血葡萄球菌、人葡萄球菌和沃氏葡萄球菌中的比例分别为80.36%、68.42%、90.00%、81.82%和54.55%;诱导型克林霉素耐药检出率在表皮葡萄球菌、金黄色葡萄球菌、溶血葡萄球菌、人葡萄球菌和沃氏葡萄球菌中的比例分别为61.11%、65.00%、64.29%、42.86%和50.00%。结论凝固酶阴性葡萄球菌、金黄色葡萄球菌是医院新生儿败血症的主要病原菌;血培养检出病原菌及其耐药性是临床诊治的重要参考依据;开展D-试验以检测葡萄球菌属对红霉素和克林霉素的诱导耐药性,可帮助医师正确选用大环内酯类及林可霉素类抗菌药物。
OBJECTIVE To explore the distribution of neonatal septicemia pathogenic bacteria and drug-sensitive situation for a reasonable choice of antibiotics.METHODS VersaTREK-240 automated blood culture system was applied for the culture of blood samples and ARIS 2X micro-analysis system was applied for bacteriological identification and the drug sensitivity analysis was performed.RESULTS A total of 159 strains of pathogens were isolated from 890 cases of blood culture,among which Gram-positive cocci accounted for 94.97%,Gram-negative bacilli accounted for 4.40%;fungi accounted for 0.63%.CNS was the major pathogen,followed by S.aureuse and Streptococcus.The drug resistant rates of Staphylococcus to PEN,AMP,OXA and ERY were high.Vancomycin-resistant strains were not found in Gram-positive bacteria.The detection rates of methicillin-resistant Staphylococcus in S.epi,S.aureuse,S.hae,S.hom and S.war were 80.36%,68.42%,90.00%,81.82% and 54.55%,respectively Inducible clindamycin resistance detection rate of S.epi,S.aur,S.hae、S.hom and S.war in the proportion respectively 61.11%,65.00%,64.29%,42.86 % and 50.00%.CONCLUSION CNS and S.aureuse are the major hospital pathogens causing the neonatal sepsis.To detect the pathogens from the blood culture and their drug resistance is an important reference for the clinical diagnosis and treatment.Carrying out D-test to detect the drug resistance of staphylococcus to erythromycin and clindamycin can help the physicians to select the right type of lincomycin and macrolide antibiotics.
出处
《中华医院感染学杂志》
CAS
CSCD
北大核心
2011年第3期603-605,共3页
Chinese Journal of Nosocomiology
关键词
新生儿
败血症
药物敏感率
诱导耐药性
Neonate
Sepsis
Drug sensitive rate
Induced drug resistance
