摘要
目的：建立测定车前草中高车前甙含量的高效液相色谱法。方法：以Ｎｕｃｌｅｏｓｉｌ７Ｃ１８（４．６×２５０ｍｍ，１０μ）为分析柱，流动相为甲醇水（ｐＨ３．５，１．０ｍｏｌ／Ｌ）磷酸盐缓冲液（４０∶５５∶５）；柱温４５℃；检测波长：３３５ｎｍ；流速：１．０ｍＬ／ｍｉｎ，采用面积外标法。结果：线性范围６．２５×１０－３～０．２０μｇ，平均回收率为１００．８％，ＲＳＤ为１．１９％（ｎ＝９）。结论：本法简便、灵敏、重现性好，可用于车前草中高车前甙含量测定。
OBJECTIVE: To eatablish a HPLC method for the determination of homoplantaginin in Herba Plantaginis. METHODS: Analysis column of Nucleosil7 C 18 (4.6×250mm,10μ), mobile phase of methanol water 1.0mol/L, pH3.5 phosphate buffer (40∶55∶5), column temperature at 45℃, detective wavelength at 335nm, 1.0mL/min of flow rate and external standard method. RESULTS: The method was linear within the range of 6.25×10 -3 -0.20μg, the average recovery was 100.8%, and the RSD was 1.19%(n=9). CONCLUSION: The method is simple, sensitive, and highly reproducible, and may be used for the quantitative determination of homoplantaginin in Herba Plantaginis.
出处
《中成药》
CAS
CSCD
1999年第7期370-372,共3页
Chinese Traditional Patent Medicine
