摘要
目的探讨米托蒽醌（ＭＸＲ７）基因在人肝癌组织中的表达及意义。方法以ＭＸＲ７基因ｃＤＮＡ全长的ＰＣＲ产物经核素［α３２Ｐ］标记、纯化后作为探针，应用Ｎｏｒｔｈｅｒｎｂｌｏｔ杂交分析，对经病理证实的术前未做治疗的３０例肝癌和癌旁肝组织、１２例正常肝组织中ＭＸＲ７基因的表达进行检测。结果ＭＸＲ７ｍＲＮＡ在人肝癌、癌旁肝和正常肝组织中的阳性表达率分别为７６７％、１３３％和０。在血清ＡＦＰ阴性的肝癌患者和肿瘤直径＜５ｃｍ的肝癌中，ＭＸＲ７ｍＲＮＡ阳性表达率分别为９／１０和５／６。结论ＭＸＲ７基因在人肝癌组织中的高水平表达具有普遍性与早期性，提示ＭＸＲ７基因可以作为肝癌的生物学标志物，对肝穿刺组织进行ＭＸＲ７基因表达的检测有助于发现临床上血清ＡＦＰ阴性的早期肝癌。
Objective To discuss the proper opportunity of surgery for rectal cancer after preoperative intra aterial chemotherapy. Methods Preoperative chemotherapy was performed on 27 rectal cancer cases at Dukes stage B and C. The therapy comprised of arterial infusion of 5 fluorouracil 600 mg/m 2, mitomycin 15 mg/m 2 and epirubicin 30 mg/m 2 for one course. Miles′ or Dixon′s operation was carried out 7 to 10 days later. The effects of chemotherapy were evaluated. Dynamic changes of proliferating cell nuclear antigen (PCNA) expression were studied by immunohistochemistry on paraffin embedded sections. Results Hematochezia was remittes in 19 cases. Histologically, the effects of chemotherapy were considered as slight, moderate and marked in 9,15 and 3 cases, respectively. Side effects were slight and recovered in 4 days. There was a high expression of PCNA in rectal cancer.On the 7th to the 10th day,proliferating index was significantly higher than that of before chemotherapy(46 48±10 62)%, P <0 05. Conclusions Preoperative adjuvant chemotherapy plays a role in the treatment of resectable rectal cancer. It is suggested that radical surgery should be carried out 5 to 6 days after intra aterial chemotherapy.
出处
《中华外科杂志》
CAS
CSCD
北大核心
1999年第3期171-173,I010,共4页
Chinese Journal of Surgery
基金
国家自然科学基金
美国Sugen高技术公司基金
关键词
肝肿瘤
基因表达
米托蒽醌
MXR7
Rectal neoplasms Adenocarcinoma Drug therapy,combination Proliferating cell nuclear antigen
