摘要
多元药物抗性基因(multidrug resistance gene,mdr-1)编码 P 170膜蛋白,可使细胞对多种结构不同的细胞毒药物产生抗性。本文以 mdr-1 cDNA为探针,做白细胞总 RNA 斑点杂交分析了5例髓性白血病人mdr-1的mRNA水平。说明mdr-1基因在此类患者的白细胞中的表达有明显差异,同一病人治疗前后也有改变。所建立的观查临床标本中基因表达水平的方法,具广泛的应用价值。
P-glycoprotein (Pgp; Mr=170,000) is encoded by a family of genes-Multidrug resistance gene.The Pgp bas been demonstrated to mediate resistance to multiple structurally dissimilar drugs, whichfuctions as an energy-dependent efflux pump so that a cell with high level of mdr expression canmore effectively eliminate cytotoxic drugs. In this report, a simplified method for analysis of clinicalsamples and assess the level of gene expression was set up. Furthermore, by using ^(32)P labelled mdr-1cDNA as the probe and RNA dot blotting the mdr-mRNAs from 5 cases of myeloblastic leukemiacells were analysed. It was shown that the level of mdr-1 expression in different myeloleukemic cellswas various and reduced in one case after remission. The established method for mRNA analysis couldbe generalized for evaluating the level of mRNA in clinical samples.
出处
《北京医科大学学报》
CSCD
1992年第3期165-167,共3页
Journal of Peking University(Health Sciences)
基金
国家自然科学基金
