摘要
本实验菌株醋酸钙不动杆菌(Acinetobacter calcoaceticus)M1-7的最佳紫外诱变时间为60,s.利用紫外诱变后涂布到添加不同脂肪酶底物和分解产物的分离培养基,筛选出具有抗性的正突变株.其中柠檬酸钠抗性突变菌株UN9最高酶活力达15.960,U/mL,比出发菌株提高了130.97%,远远高于琥珀酸钠、正丁酸、正己酸、三丁酸甘油酯等抗性突变菌株.经5次传代后,菌株UN9遗传性能稳定,平均酶活力为15.866,U/mL.
The best time for UV-mutation of Acinetobacter calcoaceticus M1-7 is 60 s.And the target resistant mutants are screened on media containing lipase substrate and product.The highest enzyme activity identified from a sodium citrate resistant mutant strain UN9 is 15.960 U/mL,a yield increase of 130.97% compared with the starting strain,far higher than those from resistant mutants to other classes,such as sodium succinate,butyrate acid,caproic acid and butyrins.The genetic stability of strain UN9 is high based on results from five generations of propagation,and its average lipase activity is 15.866 U/mL.
出处
《天津科技大学学报》
CAS
2010年第6期1-4,共4页
Journal of Tianjin University of Science & Technology
基金
天津市自然科学基金资助项目(09JCZDJC17800)
关键词
脂肪酶
醋酸钙不动杆菌
紫外诱变
抗性突变菌株
酶活力
lipase
Acinetobacter calcoaceticus
UV-mutation
resistant mutant strain
enzyme activity
