摘要
以高胡萝卜素自交系P50006和HCM A.C.为亲本构建的F2群体为作图群体,对胡萝卜中α-胡萝卜素、α-胡萝卜素、总胡萝卜素和番茄红素含量进行QTL定位及遗传分析。结果表明,α、β-胡萝卜素、总胡萝卜素和番茄红素含量的广义遗传力分别为0.75、0.50、0.31和0.93。遗传图谱包含91个SRAP(Sequence-related amplified polymorphism)标记,分布于9个连锁群,总长度502.9cM,标记间平均距离5.5cM。除α-胡萝卜素含量外,α-胡萝卜素、总胡萝卜素和番茄红素含量分别检测到1个主效QTL,均为加性遗传效应,分别解释表型变异为12.79%、12.87%和14.61%。此外,α-胡萝卜素和番茄红素含量还分别检测到1对上位性QTL,最大遗传效应分别为显性×加性互作和显性×显性互作,分别解释表型变异为15.1%和6.5%。文章中与QTL连锁的分子标记可用于高胡萝卜素、番茄红素的种质筛选和聚合育种。
An F2 population derived from two carrot inbred lines,P50006 and HCM A.C. with high carotene accumulation,was developed and used to map and analyze quantitative trait locus（QTL） associated with the accumulation of α and β-carotene,total carotene and lycopene. Broad-sense heritabilities of these traits were 0.75,0.50,0.31,and 0.93,respec-tively. A genetic map with 91 SRAP（Sequence-related amplified polymorphism） markers was developed,which spanned 502.9 cM in 9 linkage groups with a mean marker interval of 5.5 cM. Mixed-model-based composite interval mapping was performed to analyze QTL and epistasis effects. One major QTL each for α-carotene,total carotene and lycopene accumula-tion were detected which can explain 12.79%,12.87%,and 14.61% of total phenotypic variations,respectively. Additive genetic variance was primarily responsible for genetic variability in all three major QTL. In addition,a pair of epistasis QTL for α-carotene and lycopene accumulation was detected,which were able to explain 15.1% and 6.5% of total phenotypicvariation,respectively. The dominant × additive and dominant × dominant interaction variance were primary epistasis effect for α-carotene and lycopene. These SRAP markers linked to QTL could be used in selection or QTL pyramiding for high carotene and lycopene content in carrot breeding.
出处
《遗传》
CAS
CSCD
北大核心
2010年第12期1290-1295,共6页
Hereditas（Beijing)
基金
国家科技支撑项目(编号:2006BAD13B06)
农业部948项目(编号:2006-G13)
中央级公益性科研院所基本科研业务费专项(编号:2060302-2-09)
公益性行业(农业)科研专项(编号:200903016)资助
