摘要
以 4 8 2× 5 0 0 3的 16 6个F2 :3家系作为定位群体 ,采用 13× 13α 简单格子方设计 ,在成都、雅安分别调查了株高、穗位高、穗长、秃尖长、穗行数、行粒数、穗粗、轴粗、粒深、30 0粒重、出籽率、小区产量等 12个经济性状 ,采用区间作图法对其进行QTL定位分析 ,共检测出 5 9个QTL ,每个性状检测出 3～ 8个QTL ,单个QTL的作用可解释表型变异的 8 4 %～4 2 2 % ,每一性状的QTL可解释表型变异的 19 5 %～ 70 9%。 5 9个QTL分布于 10个连锁群的 33个标记区域 ,其中第 1、3连锁群较多 ,分别占 2 4 2 %、15 2 % ;33个标记区域中 19个区域作用单一性状 ,6个区域分别作用两个性状 ,4个标记区域 (bnlg10 83、bnlg10 35、bnl8 4 5a、csu16 )分别作用于 3个性状 ,3个标记区域 (csu9、phi0 5 3、phi0 2 2 )分别作用于 4个性状 ,nc0 12区域同时作用于 5个性状。
F 2∶3. lines derived from a single cross between inbred lines 48-2 and 5003, together with two parents and F 1 were grown in a 13×13 α-lattice design of one-row plots with three replicates at in Chengdu and Ya'an, Sichuan Province, and evaluated for plant height, ear height, ear length,rare ear length, row number, kernel number per row, ear diameter, cob diameter, kernel depth, 300 kernel weight, grain index(%) and grain yield. With the interval mapping procedure,59 QTLs were identified for 12 quantitative traits based on a linkage map consisting of 116 RFLP loci and 83 SSR makers. 3-8 QTLs were detected for each trait. A single QTL accounted for 8.4%-42.2% of the phenotypic variation. The total QTLs detected in each trait accounted for 19.5%-70.9% of the phenotypic variation. Partial dominance to overdominance is the primary mode of the gene action. 59 QTLs are located on 33 marker regions of the 10 linkage groups on the molecular genetic linkage map. There are more marker regions on choromsome 1 and 3. 19 marker regions have only effects on single trait. 6 marker regions have effects on two traits. The regions of bnlg1083, bnlg1035, bnl8.45a and csu16 affect three traits respectively,while the regions of csu9,phi053 and phi022 control four traits respectively. The nc012 region affects five traits.
出处
《作物学报》
CAS
CSCD
北大核心
2005年第2期188-196,共9页
Acta Agronomica Sinica
基金
四川省"十五"农业生物技术育种攻关项目
国家"863"项目"玉米分子标记辅助育种技术与优质
高产多抗新品种选育"(2003AA207070)
四川省"十五"玉米育种及种质资源研究项目资助
