摘要
利用单链构象多态性(SSCP),建立微流控芯片电泳(ME)联合激光诱导荧光检测(LIF)技术,检测人类p53基因点突变的方法。设计不同碱基长度的p53单链序列,针对易突变的外显子7,8,9进行SSCP分析,分离野生与突变的单链DNA序列;研究了筛分介质聚乙烯基氧化物(PEO)的浓度,场强对芯片电泳行为的影响。在PEO质量分数为0.5%,分离场强为260V/cm时,100 s之内就可以实现样品p53外显子7,8,9的野生型与突变型碱基的分离检测。
This study examined the potential of microchip electrophoresis with a laser-induced fluorescence detector using a conventional glass microchip for the fast detection and simultaneous analysis of mutations in the p53 gene of exon 7,8 and 9.The separation efficiency at various polyethyleneoxide concentrations and the injection electric field were investigated.The mutant and normal ssDNA in p53 gene were analyzed within 100 s under the optimized conditions.The microchip electrophoresis with a laser-induced fluorescence detector technique is a powerful tool for the fast detection and simultaneous analysis of p53 point mutations.
出处
《分析试验室》
CAS
CSCD
北大核心
2010年第12期1-4,共4页
Chinese Journal of Analysis Laboratory
基金
教育部新世纪人才支持计划(NECT-08-0913)
上海市科委项目(08142201400)资助
