摘要
以黄芪种子和带子叶的上胚为处理对象,用在培养基里添加不同浓度秋水仙素的方法,研究了秋水仙素不同浓度、不同处理时间对黄芪多倍体诱导的影响.结果表明,两种处理对象的诱导效果受秋水仙素浓度和处理时间两方面的影响.以成熟种子为处理对象,各种秋水仙素浓度和处理时间组合均可诱导出多倍体植株,但以秋水仙素浓度100mg/L,处理时间14d的组合加倍率最高,达13.3%;以带子叶的上胚为处理对象,从秋水仙素浓度50mg/L,处理时间7d开始有较低的加倍率,以秋水仙素浓度100mg/L处理时间7d的加倍效果最好,为10.9%.细胞染色体鉴定结果为:四倍体染色体数为2n=4x=32;而二倍体的染色体数为2n=2x=16.
Astragalus mernbranaceus （Fisch.） Bge. var. mongholicus（Bge. ） Hsiao seeds and embryos with cotyledons were treated by adding different concentrations of colchicines to culture medium. The effects of the different colchicine concentrations and different treatment times on the induction of polyploid Astragalus membranaceus（Fisch.） Bge. var. mongholicus（Bge. ） Hsiao are studied. The results show that the effects of the two induced objects are affected by the colchicine concentration and treatment time. For mature seeds,polyploid plants can be induced by various combinations of colchicine concentration and treatment time,and 13.3 % doubling rate is obtained by treating with the combination of colchicine concentration 100mg/L,treatment time 14d;For the embryos with cotyledons,using the colchicine concentration 50mg/L and treatment time 7d,the embryos begin to lower doubling rate;the best result of 10.9% is obtained by using the combination of colcbicine concentration 100mg/L and the treatment time 7d. Chromosome identification results are that the tetraploid chromosome number is 2n=4x=32 and the diploid chromosome number is 2n= 2x=16.
出处
《内蒙古大学学报（自然科学版）》
CAS
CSCD
北大核心
2010年第6期683-688,共6页
Journal of Inner Mongolia University：Natural Science Edition
基金
内蒙古自然科学基金资助项目(200607010101)
关键词
蒙古黄芪
秋水仙素
多倍体诱导
Astragalus rnembranaceus（Fisch. ） Bge. var. mongholicus（Bge. ） Hsiao colchicine polyploidy induction
