摘要
对N+注入紫花苜蓿Medicago sativa所引起生物学效应从生理生化层面进行了较系统的研究,结果显示:在低剂量范围内(0~2.08×1016N+/cm2),N+注入对紫花苜蓿种子存在当代刺激效应,所研究的几种生理生化指标相对CK都有所提高。剂量为6.24×1016~8.32×1016N+/cm2的N+注入对紫花苜蓿种子存在反常辐照损伤效应。即随着N+注入剂量增加,各生理生化指标先降,后升,再降。N+注入使紫花苜蓿过氧化物酶同工酶酶谱发生变异。处理组与对照组扩增出的相同谱带亦存在谱带荧光强度有差异的现象。另外,对N+注入束介导大豆基因组DNA转入紫花苜蓿做了初步研究,结果M2总性状突变率达到19.8%,并得到3株叶片粗蛋白含量较高的突变株(粗蛋白含量比对照高约0.5%)、1株高叶绿素含量的突变株(叶绿素含量比对照高33.3%)。
In this paper, the biological effects of transferring soy genome DNA by low energy N^＋ implantation on alfalfa were studied. The results showed that in the range of low dosage （0-2.08×10^16N^＋/cm^2）, alfalfa is stimulated by N^＋ implantation. At the range of 6.24 × 10^16 - 8.32 × 10^16 N^＋ /cm^2 , radiation damage occurred in alfalfa seeds and with the increasing dosage, most physiological andbiochemical indexes dropped firstly, then increased and followed by a second drop. N^＋ implantationcaused fluctuation of peroxides isoenzyme and peroxides isoenzyme activity. After marinated by soygenome DNA solution and ion implantation, M2 mutation rate of alfalfa was 19.8% and 3 mutantscontained higher crude protein（〉0.5%）, 1 mutant gave＇higher chlorophyll content （〉33.3 %） .
出处
《草业科学》
CAS
CSCD
2006年第1期13-17,共5页
Pratacultural Science
基金
湖南科技大学博士基金项目(E54141)
湖南省教育厅基金项目
关键词
紫花苜蓿
N^+注入
生理生化指标
离子束介导转基因
alfalfa
N^＋ implantation
physiological and biochemical index, gene transferring by N^＋
