摘要
根据丝氨酸/苏氨酸蛋白激酶类催化结构域Ⅰ和Ⅷ氨基酸保守序列设计简并引物,以TcLr19、TcLr35和感病对照Thatcher的cDNA为模板进行抗病基因同源序列的PCR扩增,得到了6条通读的抗病基因同源序列(RGAs)Lr19-RGA1、Lr19-RGA2、Lr19-RGA3、Lr35-RGA1、Lr35-RGA2和TC-RGA。在NCBI中用BLASTp比对发现,Lr19-RGA1、Lr19-RGA2、Lr19-RGA3、Lr35-RGA1和Lr35-RGA2编码的氨基酸序列具有丝氨酸/苏氨酸蛋白激酶(Serine-thre-onine kinase,STK)的催化结构域Ⅱ-Ⅷ。对序列分析还发现,它们与已克隆的STK类抗病基因有不同程度的相似性,为进一步克隆小麦抗叶锈病相关基因提供了依据。
The degenerated primers were designed based on the conserved amino acid sequences of the catalytic domainⅠand Ⅷ of the Serine /Threonine（STK） protein kinase-like proteins,and six open-reading of disease resistance gene analogs（RGAs） named Lr19-RGA1,Lr19-RGA2,Lr19-RGA3,Lr35-RGA1,Lr35-RGA2 and TC-RGA were obtained by PCR using cDNA as templates extracted from wheat TcLr19,TcLr35 and Thatcher. All catalytic domainsⅡ-Ⅷ of STK protein kinase were found in the deduced amino acid of Lr19-RGA1,Lr19-RGA2,Lr19-RGA3,Lr35-RGA1 and Lr35-RGA2 after aligned in the NCBI with BLASTp. Homology analysis indicated that they all have different homologies to STK resistance genes that have cloned. These results provide the basis for further study on cloning the STK resistance genes in wheat.
出处
《华北农学报》
CSCD
北大核心
2010年第5期20-24,共5页
Acta Agriculturae Boreali-Sinica
基金
河北省自然科学基金(C2008000281)
国家自然科学基金(30700505)
河北省教育厅项目(Z2007408)
