摘要
目的:运用电子克隆的方法获得小麦中的CPP转录因子基因。方法:以水稻的CPP转录因子作为探针,对小麦的EST数据库进行搜索,应用相关软件进行聚类分析、拼接组装和延长。结果:克隆出4个小麦CPP基因,分别命名为TaCPP1、TaCPP2、TaCPP3和TaCPP4,序列长度分别为977bp、3 022bp、1 582bp和1 156bp,开放阅读框为975bp、2 298bp、720bp和750bp。4个CPP类型蛋白质都具有一个或两个CXC域,而且多数还拥有完整的CRC结构域。结论:4个小麦CPP基因与水稻CPP蛋白具有高度的同源性。研究结果为进一步实验克隆和研究其功能提供了理论基础。
Objeclive：CPP genes from wheat were cloned in silico cloning. Method：4 CPP genes were cloned by blasting the EST database and using the bioinformatics soft with the rice CPP as a querying probe. Result：In this study,bioinformatics methods based on EST identi- fied 4 CPP genes in wheat which were named TaCPP1, TaCPP2, TaCPP3 and TaCPP4 respectively. The cDNA length of 4 wheat CPP genes were 977bp,3 022bp,1 582bp and 1 156bp,whieh included 975bp,2 298bp,720bp and 750bp open reading frame（ORF） respec- tively. Finally,the alignment of amino acid sequences suggested that every CPP- like protein had one or two CXC domains and most also had a complete CRC domain. Condusion：The phylogenetic tree suggested that the CPP transcription factors in wheat were near in relation- ship with the ones in rice, which provided the basis for predicting their fimetions. These results provided theory basis for the gene cloning by PCR technique and functional analysis.
出处
《生物技术》
CAS
CSCD
北大核心
2014年第4期39-42,共4页
Biotechnology
基金
河南科技大学人才基金项目("作物抗逆相关转录因子家族新基因的筛选与功能鉴定"
No.09001407)资助
关键词
小麦
CPP基因
电子克隆
生物信息学
Triticum aestivum L
CPP protein gene
Electronic clone
Bioinformaties
