摘要
应用RT-PCR法从甘蔗(Saccharum L.)花穗中克隆了1个丝氨酸精氨酸丰富蛋白(Serine/Arginine-rich proteins,SR proteins)家族基因ScRS31,属于RS亚家族成员。该序列全长1 120 bp,包含编码285个氨基酸的完整开放读码框,具有典型的RS亚家族结构特征。生物信息学分析显示,ScRS31编码蛋白是一个不稳定蛋白,表现亲水性;含有41个潜在丝氨酸磷酸化位点,推测通过磷酸化和去磷酸化实现功能调控;与高粱、玉米中的SR蛋白氨基酸序列的同源性最高,分别为97.5%、96.1%。实时荧光定量分析发现,ScRS31在根、花和芽中的表达量远高于其它组织中该基因的表达量。
A serine/arginine-rich gene,ScRS31,which belonged to RS subfamily,was cloned by RT-PCR from the inflorescence of sugarcane.It was 1 120 bp long with an ORF encoding 285 putative amino acids,and had the typical RS subfamily genetic structure.The bioinformetic analysis suggested the putative ScRS31 protein was hydrophilic and instable.It had 41 potential phosphorylation sites,suggesting that it could carry out functional regulation by phosphorylation and dephosphorylation.Homology analysis showed ScRS31 protein had high homology to sorghum and maize,which similarity to them was 97.5％ and 96.1％,respectively.The result of real-time PCR showd that the expression of ScRS31 gene in root,flower and bud was much higher than that in all the other tissues.
出处
《热带作物学报》
CSCD
北大核心
2013年第10期1935-1940,共6页
Chinese Journal of Tropical Crops
基金
国家甘蔗产业技术研发体系专项资金和国家自然科学基金(No.30671329)
关键词
甘蔗
ScRS31
克隆
表达分析
Saccharum L.
ScRS31 gene
Cloning
Expression analysis
