摘要
根据GenBank上已发表的其他真菌α–微管蛋白基因序列中的保守区域设计简并引物,扩增出银耳α–微管蛋白基因的cDNA部分序列。分别利用SEFA-PCR方法和RACE技术,克隆了银耳α–微管蛋白基因的DNA和cDNA全长序列。银耳α–微管蛋白基因DNA全长1962bp,含有9个内含子,cDNA全长1347bp,编码448个氨基酸。生物信息学分析结果表明:银耳α–微管蛋白基因的cDNA序列与新型隐球酵母有80%相似性,编码的蛋白质属于Tubulin_FtsZ超家族中的α–微管蛋白亚家族,氨基酸序列中存在保守的GTP结合区域GGGTGSG,系统发育树显示银耳与新型隐球酵母距离最近。
Partial cDNA sequence of α-tubulin gene was amplified from Tremella fuciformis by degenerate primers designed according to the conserved regions of published α-tubulin sequences of other fungi on GenBank. The DNA and cDNA full length sequences of α-tubulin gene of T. fuciformis were cloned by SEFA-PCR and RACE,respectively. The DNA sequence of α-tubulin gene of T. fuciformis consisted of 1 962 bp,containing 9 introns and the cDNA sequence consisted of 1 347 bp,coding 448 amino acids. Bioinformatics analysis showed the similarity between α-tubulin cDNAs of T. fuciformis and Cryptococcus neoformans reaches to 80%,the α-tubulin of T. fuciformis belongs to α-tubulin subfamily of Tubulin_FtsZ superfamily and its amino acid sequence contains a conservative GTP binding region GGGTGSG. Phylogenetic tree revealed the evolutionary distance between T. fuciformis and C. neoformans is the nearest.
出处
《园艺学报》
CAS
CSCD
北大核心
2011年第5期921-929,共9页
Acta Horticulturae Sinica
基金
国家自然科学基金项目(30972072)
关键词
银耳
α–微管蛋白
克隆
序列分析
系统发育树
Tremella fuciformis
α-tubulin
cloning
sequence analysis
phylogenetic tree