摘要
根据丝氨酸／苏氨酸蛋白激酶类催化结构域Ⅰ和Ⅸ的氨基酸保守序列设计简并引物，PCR扩增‘紫花芒’杧果嫩绿色叶片基因组DNA。在NCBI中用Blast X比对发现，有6个阳性克隆是抗病基因同源序列，并命名为PK1～PK6，GenBank注册序列号为AY693369-AY693371和AY776277-AY776279。在PK3、PK4和PK6中发现了丝氨酸／苏氨酸蛋白激酶的9个催化结构域Ⅰ～Ⅸ，而其它3个克隆具有部分结构域。氨基酸序列同源性分析还发现，6个克隆编码的氨基酸序列都与受体样蛋白激酶有较高的氨基酸序列同源性。系统进化树分析还表明它们都是可能的抗病基因同源序列。总之，6个克隆不仅是抗病基因同源序列而且是可能的受体样蛋白激酶基因同源序列。
Based on the conserved amino acid ine/Threonine（STK）protein kinase-like proteins, sequences of the catalytic domain Ⅰand Ⅸof the Serthe degenerated primers were designed and PCR was performed on genomic DNA extracted from the peak-green leaf of mango （M. indica cv. ＇Zihuamang＇） and six DNA fragments of 500 bp were cloned and confirmed as disease resistance gene analogs （RGAs） coding STK protein kinase-like proteins after aligned in the GenBank with Blast X. They were named as PK1-PK6 with the accession number of AY693369-AY693371 and AY776277-AY776279 in the Genbank. All catalytic domains Ⅰ-Ⅸ of STK protein kinase were found in the translations of PK3, PK4 and PK6. The six clones were also found as disease resistance gene analogs by molecular phylogenic tree analysis. All of their deduced amino acids highly matched with the receptor-like protein kinase via Blast Ⅹin NCBI. It showed that the six clones were the disease resistance gene analogs and the probable receptor-like kinase gene analogs.
出处
《华中农业大学学报》
CAS
CSCD
北大核心
2006年第2期186-189,共4页
Journal of Huazhong Agricultural University
基金
中国博士后科学基金项目(2005037628)资助
关键词
杧果
蛋白激酶
抗病基因
同源序列
Mangifera indica L.
protein kinase
disease-resistant gene
analogs
