摘要
目的：利用金黄地鼠颊癌模型，探讨口腔癌发生过程中增殖细胞核抗原（ＰＣＮＡ）表达变化及其规律。方法：０．５％二甲基苯并蒽（ＤＭＢＡ）丙酮液处理地鼠颊粘膜，每周３次，３、６、９和１２周分批处死动物；对照组不处理。利用抗ＰＣＮＡ单克隆抗体，免疫组化方法（ＬＳＡＢ）检测ＰＣＮＡ的表达情况。结果：正常地鼠颊粘膜ＰＣＮＡ表达主要位于基底层和少数棘层细胞，３周单纯增生类似正常，６～９周上皮呈不同程度异常增生，ＰＣＮＡ表达扩展至棘层，甚至全层，与异常增生程度呈正相关；１２周鳞癌形成时，ＰＣＮＡ表达明显增加，且见明显异质性。结论：ＤＭＢＡ诱导地鼠颊癌发生过程中存在ＰＣＮＡ异常表达，且与癌变进展有关，提示ＰＣＮＡ表达可作为口腔癌变监测的生物学标志之一。
Objective: Using syrain hamster oral carcinogenesis model, to inverstigate PCNA expression and its role during oral cancer developing process. Methods: 0.5% DMBA (7,12-dimethylbenzanthracene) in a acetone was applied to the hamster right buccal pouch 3 times per wk for up to 12wk, control received no treatment. PCNA expression was assessed by immunohistochemically with LSAB technique. Results: During DMBA- induced hamster buccal pouch carcinogenesis, in hyperplastic epithelium (3wk), PCNA expression was similar to normal epithelium, at a moderate level in dysplasia epithelium (6～9wk) and at a very high level in squamous cell carcinoma. Further statistial analysis had showed a significant positive correlation between PCNA expression and malignant progression of oral epithelium. Conclusions: These results indicate that PCNA expression is related to DMBA-induced oral carcinogenesis and could be a useful biomarker for multistep carcinogenesis in oral cancer.
出处
《口腔医学纵横》
CAS
CSCD
1999年第2期75-77,共3页
Journal of Comprehensive Stomatology
关键词
增殖细胞核抗原
口腔癌
表达
proliferating cellnuclear antigen oral carcinogenesis syrain hamster
