摘要
Salsolinol合成酶是一种催化多巴胺(DA)和乙醛(AcH)生成Salsolinol的酶,它在内源性儿茶酚异喹啉类物质代谢过程中起重要作用。研究显示该类物质具有一定的神经毒性,与帕金森病(PD)发病机制密切相关,因此Salsolinol合成酶可能是PD的一种关键致病因子。本文从雄性SD大鼠鼠脑中分离得到Salsolinol合成酶的粗酶液,采用高效液相色谱结合电化学检测器体系(HPLC-ECD)建立了一套灵敏度高、重复性较好的酶活检测方法,并进行了方法稳定性和灵敏度的验证,方法简便、稳定,适用于不同生物样品中该酶活性的测定;同时也将该方法应用于超滤分离后不同截留分子量样品的活性测定,结果显示该酶的活性主要集中在3K下层滤液。
Salsolinol synthase is an enzyme for synthesizing salsolinol from dopamine and acetaldehyde. It is very important in the metabolic process of catechol isoquinolines such as salsolinol,N-methyl-salsolinol. The catechol isoquinolines are closely related to the pathogenesis of Parkinson ＇s disease （ PD）,it is supposed that salsolinol synthase may play a significant role in PD. In this paper,the brain of male SD rats was used for isolation. A method for purification and activity detection of salsolinol synthase with excellent sensitivity and stability were developed. The results of ultrafiltration showed that the activity of salsolinol synthase exists in low solute of 3K.
出处
《化学通报》
CAS
CSCD
北大核心
2010年第10期938-942,共5页
Chemistry
基金
国家自然科学基金项目(20435020)资助
