摘要
目的统计分析宜昌市中心人民医院2009年临床分离主要细菌耐药情况。方法细菌鉴定采用法国生物梅里埃API系统鉴定。药敏试验采用K-B法,抗菌谱遵照美国临床实验室标准化协会(CLSI)M100-S18指南。药敏结果以WHONET5.4软件进行统计分析。结果 2009年细菌室从临床共分离菌株1835株,其中革兰阴性杆菌占55.68%,革兰阳性球菌占34.05%,真菌占10.27%。大肠埃希菌和克雷伯菌属的超广谱β-内酰胺酶(ESBLs)产生率分别为68.2%和59.4%。耐甲氧西林金黄色葡萄球菌(MRSA)和耐甲氧西林凝固酶阴性葡萄球菌(MRCNS)分别占金黄色葡萄球菌(MSSA)和凝固酶阴性葡萄球菌的60.4%和65.8%。屎肠球菌的耐药率高于粪肠球菌,但分离率低于粪肠球菌。氨基糖苷类对高度耐药肠球菌的产生率为58.1%。结论宜昌市中心人民医院产ESBLs的大肠埃希菌和克雷伯菌、MRSA、MRCNS的检出率较高,特别是泛耐药的鲍曼不动杆菌的耐药率明显高于国内其他医院,应引起重视。
Objective To statistically analyze the drug-resistant status of clinical main isolates in our hospital in 2009.Methods The France bioMérieux API bacterial identification system was used to the bacterial identification.The drug susceptibility test was performed by K-B method.The antibiotics spectrum was in accordance with CLSI M100-S18 guidelines.The drug susceptibility results were statistically analyzed by WHONET5.4 software.Results 1 835 strains of bacteria were clinically isolated in the bacterial room of our hospital.Among them,Gram-negative bacteria accounted for 55.68%,Gram-positive bacteria accounted for 34.05% and fungi accounted for 10.27%.Escherichia coli and Klebsiella ultra-broad-spectrum β-lactamase productions were 68.2% and 59.4% respectively.Methicillin-resistant Staphylococcus aureus（MRSA）and methicillin-resistant coagulase negative Staphylococcus（MRCNS）accounted for 60.7% of Staphylococcus aureus and 65.8% of coagulase-negative Staphylococcus.The drug resistance rate of Enterococcus faecium was higher than that of Enterococcus faecalis,but the isolation rate of the former was lower than that of the latter.The production rate of aminoglycosides to high drug-resistant enterococci was 58.1%.Conclusion The detection rate of Escherichia coli producing ESBLs,Klebsiella,MRSA and MRCNS is higher in our hospital,especially the resistance rate of pan-resistant Acinetobacter baumannii is significantly higher than that other domestic hospitals,which should be paid more attention to.
出处
《检验医学与临床》
CAS
2010年第20期2191-2193,2195,共4页
Laboratory Medicine and Clinic
关键词
细菌鉴定
药敏试验
耐药分析
bacterial identification
drug sensitive test
analysis of drug resistance
